Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei

文献类型: 外文期刊

第一作者: Wen, Yan-Zi

作者: Wen, Yan-Zi;Lun, Zhao-Rong;Lai, De-Hua;Lun, Zhao-Rong;Zhu, Xing-Quan;Lun, Zhao-Rong;Hide, Geoff;Lun, Zhao-Rong;Hide, Geoff

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关键词: Trypanozoon;PCR-SSCP;ITS;Phylogenetic tree;Haplotype network

期刊名称:INFECTION GENETICS AND EVOLUTION ( 影响因子:3.342; 五年影响因子:3.188 )

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收录情况: SCI

摘要: The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei. (C) 2016 Elsevier B.V. All rights reserved.

分类号: R1

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