The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins

文献类型： 外文期刊

第一作者： Chao, Qing

作者： Chao, Qing;Gao, Zhi-fang;Wang, Yue-feng;Mei, Ying-chang;Zhao, Biligen-gaowa;Wang, Bai-chen;Li, Zhe;Huang, Xia-he;Wang, Ying-chun;Li, Liang;Jiang, Yu-bo

作者机构：

关键词： Maize pollen;Proteome;Phosphoproteome;Motif;Kinase;Male sterile

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Maize is unique since it is both monoecious and diclinous (separate male and female flowers on the same plant). We investigated the proteome and phosphoproteome of maize pollen containing modified proteins and here we provide a comprehensive pollen proteome and phosphoproteome which contain 100,990 peptides from 6750 proteins and 5292 phosphorylated sites corresponding to 2257 maize phosphoproteins, respectively. Interestingly, among the total 27 overrepresented phosphosite motifs we identified here, 11 were novel motifs, which suggested different modification mechanisms in plants compared to those of animals. Enrichment analysis of pollen phosphoproteins showed that pathways including DNA synthesis/chromatin structure, regulation of RNA transcription, protein modification, cell organization, signal transduction, cell cycle, vesicle transport, transport of ions and metabolisms, which were involved in pollen development, the following germination and pollen tube growth, were regulated by phosphorylation. In this study, we also found 430 kinases and 105 phosphatases in the maize pollen phosphoproteome, among which calcium dependent protein kinases (CDPKs), leucine rich repeat kinase, SNF1 related protein kinases and MAPK family proteins were heavily enriched and further analyzed. From our research, we also uncovered hundreds of male sterility-associated proteins and phosphoproteins that might influence maize productivity and serve as targets for hybrid maize seed production. At last, a putative complex signaling pathway involving CDPKs, MAPKs, ubiquitin ligases and multiple fertility proteins was constructed. Overall, our data provides new insight for further investigation of protein phosphorylation status in mature maize pollen and construction of maize male sterile mutants in the future.

分类号： Q946

  • 相关文献

[1]An integrated proteomics reveals pathological mechanism of honeybee (Apis cerena) sacbrood disease. Han, Bin,Zhang, Lan,Feng, Mao,Fang, Yu,Li, Jianke.

[2]Novel aspects of understanding molecular working mechanisms of salivary glands of worker honeybees (Apis mellifera) investigated by proteomics and phosphoproteomics. Feng, Mao,Fang, Yu,Han, Bin,Zhang, Lan,Lu, Xiaoshan,Li, Jianke.

[3]Proteome and phosphoproteome analysis of honeybee (Apis mellifera) venom collected from electrical stimulation and manual extraction of the venom gland. Li, Rongli,Zhang, Lan,Fang, Yu,Han, Bin,Lu, Xiaoshan,Zhou, Tiane,Feng, Mao,Li, Jianke,Zhang, Lan. 2013

[4]Changes of proteome and phosphoproteome trigger embryo-larva transition of honeybee worker (Apis mellifera ligustica). Gala, Alemayehu,Fang, Yu,Woltedji, Dereje,Zhang, Lan,Han, Bin,Feng, Mao,Li, Jianke.

[5]Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance. Wang, Maoyan,Gu, Dan,Liu, Tingsong,Wang, Zhaoqiang,Guo, Xiying,Hou, Wei,Bai, Yunfeng,Chen, Xiaoping,Wang, Guoying.

[6]K-Domain Splicing Factor OsMADS1 Regulates Open Hull Male Sterility in Rice. Sun Lian-ping,Zhang Ying-xin,Zhang Pei-pei,Yang Zheng-fu,Zhan Xiao-deng,Shen Xi-hong,Zhang Zhen-hua,Hu Xia,Xuan Dan-dan,Wu Wei-xun,Li Zi-he,Cao Li-yong,Cheng Shi-hua,Sun Lian-ping,Cheng Shi-hua. 2015

[7]Event-specific qualitative and quantitative PCR detection methods for Transgenic rapeseed hybrids MS1 x RF1 and MS1 x RF2. Wu, Yuhua,Wu, Gang,Xiao, Ling,Lu, Changming. 2007

[8]Characterization of maize male sterile 2 mutant by phenotypic and RNA sequencing analyses. Kang, Dan,Wang, Cuiyun,Xu, Qilong,Wang, Guoying,Liu, Yunjun.

[9]Functional analysis of a viroid RNA motif mediating cell-to-cell movement in Nicotiana benthamiana. Wang, Meng,Jiang, Dongmei,Li, Shifang,Jiang, Dongmei.

[10]Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus. Gong, Dian,Wang, Jian-Hua,Lin, Zhan-Song,Zhang, Shao-Yan,Zhang, Yu-Liang,Yu, Nai-Tong,Liu, Zhi-Xin,Gong, Dian,Lin, Zhan-Song,Zhang, Shao-Yan,Yu, Nai-Tong,Xiong, Zhongguo,Xiong, Zhongguo. 2011

[11]A combined statistical model for multiple motifs search. Guan Shan,Gao Li-Feng,Liu Xin. 2008

[12]Genome-wide identification and validation of simple sequence repeats (SSRs) from Asparagus officinalis. Li, Shufen,Li, Xu,Yuan, Jinhong,Deng, Chuanliang,Gao, Wujun,Zhang, Guojun,Wang, Lianjun.

[13]Bioinformatics Analysis of NBS-LRR Encoding Resistance Genes in Setaria italica. Zhao, Yan,Weng, Qiaoyun,Song, Jinhui,Ma, Hailian,Yuan, Jincheng,Liu, Yinghui,Dong, Zhiping.

[14]Genome-wide analysis suggests divergent evolution of lipid phosphotases/phosphotransferase genes in plants. Wang, Peng,Chen, Zhenxi,Gai, Jiangtao,Wang, Peng,Chen, Zhenxi,Gai, Jiangtao,Kasimu, Rena,Chen, Yinhua,Zhang, Xiaoxiao.

[15]The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis. Zhang, Chunyan,Sun, Wen,Dong, Mengmeng,Liu, Wanquan,Li, Lu,Xu, Zhuofei,Zhou, Rui,Tan, Meifang,Gao, Ting,Li, Lu,Xu, Zhuofei,Zhou, Rui. 2017

[16]A comparative analysis of phosphoproteome in ovine muscle at early postmortem in relationship to tenderness. Li, Xin,Chen, Lijuan,He, Fan,Li, Meng,Zhang, Dequan,Shen, Qingwu. 2017

[17]A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.). Qiu, Jiehua,Hou, Yuxuan,Wang, Yifeng,Li, Zhiyong,Zhao, Juan,Tong, Xiaohong,Lin, Haiyan,Wei, Xiangjin,Zhang, Jian,Lin, Haiyan,Ao, Hejun. 2017

[18]Phosphoproteomic Analysis of Protein Phosphorylation Networks in the Hypopharyngeal Gland of Honeybee Workers (Apis mellifera ligustica). Qi, Yuping,Fan, Pei,Hao, Yue,Han, Bin,Fang, Yu,Feng, Mao,Cui, Ziyou,Li, Jianke,Fan, Pei,Cui, Ziyou,Cui, Ziyou.

[19]In-Depth Phosphoproteomic Analysis of Royal Jelly Derived from Western and Eastern Honeybee Species. Han, Bin,Fang, Yu,Feng, Mao,Lu, Xiaoshan,Huo, Xinmei,Meng, Lifeng,Wu, Bin,Li, Jianke.

[20]Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers. Han, Bin,Fang, Yu,Feng, Mao,Hu, Han,Hao, Yue,Ma, Chuan,Huo, Xinmei,Meng, Lifeng,Zhang, Xufeng,Wu, Fan,Li, Jianke.

作者其他论文 更多>>

微信小程序