A novel M2e-multiple antigenic peptide providing heterologous protection in mice

文献类型： 外文期刊

第一作者： Wen, Feng

作者： Wen, Feng;Ma, Ji-Hong;Yu, Hai;Yang, Fu-Ru;Huang, Meng;Zhou, Yan-Jun;Li, Ze-Jun;Wang, Xiu-Hui;Li, Guo-Xin;Jiang, Yi-Feng;Tong, Wu;Tong, Guang-Zhi

作者机构：

关键词： H3N2;M2e-multiple antigenic peptide;high-yield;inactivated vaccine;swine influenza virus

期刊名称：JOURNAL OF VETERINARY SCIENCE （ 影响因子：1.672； 五年影响因子：1.904 ）

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年卷期：

页码：

收录情况： SCI

摘要： Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.

分类号： S8

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