文献类型: 外文期刊
第一作者: Wen, Feng
作者: Wen, Feng;Ma, Ji-Hong;Yu, Hai;Yang, Fu-Ru;Huang, Meng;Zhou, Yan-Jun;Li, Ze-Jun;Wang, Xiu-Hui;Li, Guo-Xin;Jiang, Yi-Feng;Tong, Wu;Tong, Guang-Zhi
作者机构:
关键词: H3N2;M2e-multiple antigenic peptide;high-yield;inactivated vaccine;swine influenza virus
期刊名称:JOURNAL OF VETERINARY SCIENCE ( 影响因子:1.672; 五年影响因子:1.904 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
分类号: S8
- 相关文献
作者其他论文 更多>>
-
An Expeditious Neutralization Assay for Porcine Reproductive and Respiratory Syndrome Virus Based on a Recombinant Virus Expressing Green Fluorescent Protein
作者:Wang, Juan;Yan, Jiecong;Wang, Shuaiyong;Chen, Ronglin;Xing, Yanru;Liu, Qingyan;Gao, Shuolei;Zhu, Yuxiang;Li, Jiannan;Zhou, Yanjun;Shan, Tongling;Tong, Wu;Zheng, Hao;Kong, Ning;Jiang, Yifeng;Liu, Changlong;Tong, Guangzhi;Yu, Hai;Tong, Guangzhi;Yu, Hai
关键词:PRRSV; infectious clones; reporter virus; enhanced GFP; neutralizing antibodies; neutralization assay
-
Development and application of a triplex real-time PCR assay for the detection of H3, H4, and H5 subtypes of avian influenza virus
作者:Wen, Feng;Wang, Congying;Guo, Jinyue;Yuan, Sheng;Li, Zhili;Huang, Shujian;Yu, Hai;Li, Yong;Liang, Zhaoping
关键词:avian influenza virus; H3; H4; H5; TaqMan probe
-
Recent H9N2 avian influenza virus lost hemagglutination activity due to a K141N substitution in hemagglutinin
作者:Wen, Feng;Yan, Zhanfei;Chen, Gaojie;Chen, Yao;Wang, Nina;Li, Zhili;Guo, Jinyue;Liu, Quan;Huang, Shujian;Wen, Feng;Yu, Hai
关键词:avian influenza virus; hemagglutination activity; haemagglutinin; mutation; thermostability; surveillance; poultry
-
RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway
作者:Wang, Xiaoquan;Zhang, Zhendong;Wang, Xiaoquan;Tong, Wu;Yang, Xinyu;Zhai, Huanjie;Qin, Wenzhen;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Kong, Ning;Shan, Tongling;Tong, Wu;Yang, Xinyu;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Kong, Ning;Shan, Tongling
关键词:RBM14; PEDV; N protein; p62; autophagy; IFN
-
Genomic variation in weedy and cultivated broomcorn millet accessions uncovers the genetic architecture of agronomic traits
作者:Lu, Qiong;Zhao, Hainan;Zhang, Zhengquan;Bai, Yuhe;Zhao, Haiming;Zheng, Yunxiao;Zhao, Haiyue;Gong, Huihui;Chen, Lingwei;Deng, Xizhen;Hong, Xiangde;Liu, Tianxiang;Li, Baichuan;Bai, Yan;Xin, Beibei;Chen, Jian;E., Lizhu;Lai, Jinsheng;Song, Weibin;Zhao, Hainan;Lai, Jinsheng;Song, Weibin;Liu, Guoqing;Li, Haiquan;Liu, Minxuan;Lu, Ping;Wen, Feng;Wang, Lun;Li, Zhijiang;Li, Hai;Zhang, Like;Ma, Wenhui;Liu, Chunqing;Bai, Yan;Lai, Jinsheng;Song, Weibin;Lai, Jinsheng;Song, Weibin
关键词:
-
PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein
作者:Zhai, Xueying;Yang, Xia;Zhai, Xueying;Kong, Ning;Wang, Chunmei;Qin, Wenzhen;Dong, Sujie;Zhai, Huanjie;Yang, Xinyu;Ye, Chenqian;Ye, Manqing;Li, Guoxin;Tong, Wu;Liu, Changlong;Zheng, Hao;Yu, Hai;Tong, Guangzhi;Shan, Tongling;Zhang, Wen
关键词:MARCH8; N protein; NDP52; PEDV; PRPF19; selective autophagy
-
Reduced glutathione and raffinose lengthens postharvest storage of cassava root tubers by improving antioxidant capacity and antibiosis
作者:Fu, Haitian;Zhao, Ying;Huang, Jianqi;Luo, Yanchun;Wei, Zusheng;Yu, Benchi;Wen, Feng;Fu, Haitian;Zhao, Ying;Huang, Jianqi;Luo, Yanchun;Wei, Zusheng;Yu, Benchi;Wen, Feng
关键词:Cassava; GR15231; Raffinose; Reduced glutathione; SC14; Storage tolerance