Development of gene-based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa

文献类型： 外文期刊

第一作者： Li, Guo-Liang

作者： Li, Guo-Liang;Qian, Wei;Zhang, Shu-Jiang;Zhang, Shi-Fan;Li, Fei;Zhang, Hui;Wu, Jian;Wang, Xiao-Wu;Sun, Ri-Fei

作者机构：

关键词： Brassica rapa;Turnip mosaic virus;dCAPS;retr02;molecular marker-assisted selection;Kompetitive Allele Specific PCR

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

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收录情况： SCI

摘要： Turnip mosaic virus (TuMV) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of TuMV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence (dCAPS) marker and a Kompetitive Allele Specific PCR (KASP) marker were developed based on a single recessive gene, retr02, which confers broad-spectrum TuMV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost-effective and quick to process, which is likely to be beneficial in establishing high-throughput assays for marker-assisted selection.

分类号： S3

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