Development of an HRM-based, safe and high-throughput genotyping system for two low phytic acid mutations in soybean

文献类型： 外文期刊

第一作者： Tan, Yuan-Yuan

作者： Tan, Yuan-Yuan;Shu, Qing-Yao;Shi, Chun-Hai;Yu, Xiao-min;Yuan, Feng-Jie;Zhang, Hua-Li;Wang, Shou-Gen

作者机构：

关键词： Soybean;Low phytic acid;High-resolution melting curve analysis;GmMIPS1;GmIPK1

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Two low phytic acid (lpa) mutants, Gmlpa- ZC-2 (ZC-lpa) and Gm-lpa-TW-1 (TW-lpa), resulting from a G -> A mutation in GmIPK1 and a 2-bp deletion in GmMIPS1, respectively, were previously developed to increase the nutritional value and environmental friendliness of soybean meal. Two functional CAPS markers were subsequently developed for genotyping plants carrying the two mutant genes; however, both are costly and time consuming and hence unsuitable for large-scale breeding use. In the present work, by integrating a quick DNA extraction protocol with an optimized high-resolution melting curve (HRM) analysis, we developed a fast and high-throughput genotyping system for the two mutations. In this system, (1) DNAs are extracted within half an hour using a protocol that only requires freezing and heating of leaf disks in two non-toxic solutions and can be directly used for PCR; (2) for genotyping, asymmetric PCRs with competitive primers are performed, and the samples are then discriminated and grouped through HRM analysis; and (3) all steps are performed in a 96-well plate, and hence adaptable to high-throughput genotyping. Although the system was developed for two lpa mutations, the general principle should be applicable to any other genes in soybean.

分类号： Q94

  • 相关文献

[1]Density alteration of nutrient elements in rice grains of a low phytate mutant. Ren, Xue-Liang,Liu, Qing-Long,Fu, Hao-Wei,Wu, Dian-xing,Shu, Qing-Yao. 2007

[2]Genetic diversity and aggressiveness of Fusarium species isolated from soybean in Alberta, Canada. Zhou, Qixing,Chang, Kan-Fa,Hwang, Sheau-Fang,Fu, Heting,Turnbull, George D.,Li, Nana,Strelkov, Stephen E.,Conner, Robert L.,McLaren, Debra L.,Harding, Michael W.. 2018

[3]High-Density Genetic Mapping Identifies New Major Loci for Tolerance to Low-Phosphorus Stress in Soybean. Zhang, Dan,Li, Hongyan,Chu, Shanshan,Lv, Haiyan,Wang, Jinshe,Zhang, Hengyou,Hu, Zhenbin,Yu, Deyue. 2016

[4]Geographical distribution of GmTfl1 alleles in Chinese soybean varieties. Liu, Guifeng,Zhao, Lin,Qiu, Lijuan,Liu, Ying,Chang, Ruzhen,Guan, Rongxia,Qiu, Lijuan,Averitt, Benjamin J.,Zhang, Bo,Ma, Yansong,Luan, Xiaoyan. 2015

[5]Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. Li, Pan-Song,Chai, Shou-Cheng,Li, Pan-Song,Yu, Tai-Fei,He, Guan-Hua,Chen, Ming,Zhou, Yong-Bin,Xu, Zhao-Shi,Ma, You-Zhi. 2014

[6]Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability. Song, Zhang-yue,Tian, Jing-luan,Fu, Wei-zhe,Li, Lin,Lu, Ling-hong,Zhou, Lian,Shou, Hui-xia,Shan, Zhi-hui,Tang, Gui-xiang. 2013

[7]A CIB1-LIKE transcription factor GmCIL10 from soybean positively regulates plant flowering. Yang DeGuang,Zhao Wang,Meng YingYing,Li HongYu,Liu Bin. 2015

[8]Stability of growth periods traits for soybean cultivars across multiple locations. Liu Zhang-xiong,Chang Ru-zhen,Qiu Li-juan,Wang Xiao-bo,Yang Chun-yan,Xu Ran,Zhang Li-feng,Lu Wei-guo,Wang Qian,Wei Su-hong,Yang Chun-ming,Wang Hui-cai,Wang Rui-zhen,Zhou Rong,Chen Huai-zhu. 2016

[9]Purification and characterization of beta-glucosidase from newly isolated Aspergillus sp MT-0204. Qi, Bin,Liu, Xianjin,Qi, Bin,Wang, Limei. 2009

[10]The Soybean Basic Helix-Loop-Helix Transcription Factor ORG3-Like Enhances Cadmium Tolerance via Increased Iron and Reduced Cadmium Uptake and Transport from Roots to Shoots. Xu, Zhaolong,Liu, Xiaoqing,He, Xiaolan,Xu, Ling,Huang, Yihong,Shao, Hongbo,Zhang, Dayong,Shao, Hongbo,Tang, Boping,Ma, Hongxiang. 2017

[11]Aspects of soybean insect resistance breeding in China. Wang, S. 2004

[12]QTL Mapping of Isoflavone, Oil and Protein Contents in Soybean (Glycine max L. Merr.). Liang Hui-zhen,Yu Yong-liang,Wang Shu-feng,Lian Yun,Wang Ting-feng,Wei Yan-li,Gong Peng-tao,Fang Xuan-jun,Liu Xue-yi,Zhang Meng-chen. 2010

[13]RNAi-mediated SMV P3 cistron silencing confers significantly enhanced resistance to multiple Potyvirus strains and isolates in transgenic soybean. Yang, Xiangdong,Niu, Lu,Zhang, Wei,Yang, Jing,Xing, Guojie,He, Hongli,Guo, Dongquan,Du, Qian,Qian, Xueyan,Yao, Yao,Li, Qiyun,Dong, Yingshan. 2018

[14]Genome-wide identification and expression analysis of the CPP-like gene family in soybean. Zhang, L.,Wang, Y. M.,Yuan, C. P.,Zhang, Y. Y.,Li, H. Y.,Dong, Y. S.,Zhao, H. K.,Yan, X. F.,Li, Q. Y.. 2015

[15]Optimal Concentration of Zinc Sulfate in Foliar Spray to Alleviate Salinity Stress in Glycine soja. Jiang, W.,Xu, H. L.,Lu, H. F.,Jiang, W.,Sun, X. H.,Mantri, N.. 2014

[16]Allelism and molecular mapping of soybean necrotic root mutants. Palmer, Reid G.,Zhang, Lei,Huang, Zhiping. 2008

[17]Detection of Hirsutella spp. and Pasteuria sp parasitizing second-stage juveniles of Heterodera glycines in soybean fields in China. Ma, R,Liu, XZ,Jian, H,Li, SD. 2005

[18]Fine mapping and identification of the soybean R-SC4 resistance candidate gene to soybean mosaic virus. Wang, Dagang,Ma, Ying,Liu, Ning,Yang, Zhonglu,Zheng, Guijie,Zhi, Haijian,Wang, Dagang. 2011

[19]Comparative transcriptome analysis of soybean response to bean pyralid larvae. Sun, Zudong,Cai, Zhaoyan,Chen, Huaizhu,Lai, Zhenguang,Yang, Shouzhen,Tang, Xiangmin. 2017

[20]Association of extracellular dNTP utilization with a GmPAP1-like protein identified in cell wall proteomic analysis of soybean roots. Wu, Weiwei,Lin, Yan,Liu, Pandao,Chen, Qianqian,Tian, Jiang,Liang, Cuiyue,Liu, Pandao. 2018

作者其他论文 更多>>

微信小程序