Preparation and heat resistance study of porcine reproductive and respiratory syndrome virus sugar glass vaccine

文献类型: 外文期刊

第一作者: Lv Fang

作者: Lv Fang;Lu Yu;Hao Zheng-lin;Zhao Yan-hong;Feng Lei;Chen Jin;Wang Li-li;Hou Ji-bo;Hao Zheng-lin;Rui Rong;Zhang li-hang;Lv Fang;Lu Yu;Zhao Yan-hong;Feng Lei;Chen Jin;Wang Li-li;Hou Ji-bo

作者机构:

关键词: Vacuum foam dry;Sugar glass;Glass transition temperature;Thermostabilization;PRRSV

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To improve the preservation period without cold-chain of the live attenuated vaccine of porcine reproductive and respiratory syndrome (PRRS), a set of thermostable formulations composed of trehalose, tryptone and other protectants were dried by vacuum foam drying (VFD) along with PRRSV solutions. In the 37 degrees C and 45 degrees C resistance ageing test, the dried foam vaccine showed significant thermostability, and the virus titer lost 0.8 Log(10) at 37 degrees C for 4 months, 1.0 Logio at 45 degrees C for 25 days. Furthermore, the foam vaccine could be stored at 25 degrees C for at least one year. Besides, the vaccine preserved in 37 degrees C, 25 degrees C and 4 degrees C for 3 months were inoculated on 20-days old piglet, and the serum titer was monitoring by ELISA kit. Inoculated two weeks later, the ELISA titer were all qualified and had the similar level compared to the commercial vaccines of the lyophilization dosage. Crown Copyright (C) 2016 Published by Elsevier Ltd. All rights reserved.

分类号: R392

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