Development of an agroinoculation system for full-length and GFP-tagged cDNA clones of cucumber green mottle mosaic virus
文献类型： 外文期刊
第一作者： Zheng, Hongying
作者： Zheng, Hongying;Xiao, Caili;Han, Kelei;Peng, Jiejun;Lin, Lin;Lu, Yuwen;Xie, Li;Chen, Jianping;Yan, Fei;Xiao, Caili;Han, Kelei;Wu, Xiaohua;Xu, Pei;Li, Guojing
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
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收录情况： SCI
摘要： The complete 6243-nucleotide sequence of a cucumber green mottle mosaic virus (CGMMV) isolate from bottle gourd in Zhejiang province, China, was determined. A full-length cDNA clone of this isolate was constructed by inserting the cDNA between the 35S promoter and the ribozyme in the binary plasmid pCB301-CH. A suspension of an Agrobacterium tumefaciens EHA105 clone carrying this construct was highly infectious in Nicotiana benthamiana and bottle gourd. Another infectious clone containing the green fluorescence protein (GFP) reporter gene was also successfully constructed. This study is the first report of the efficient use of agroinoculation for generating CGMMV infections.
分类号： R37
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