X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets

文献类型： 外文期刊

第一作者： Cai, Xuan

作者： Cai, Xuan;Zhu, Lihui;Sheng, Yongshuai;Guo, Qi;Bao, Jian;Xu, Jianxiong;Cai, Xuan;Zhu, Lihui;Sheng, Yongshuai;Guo, Qi;Bao, Jian;Xu, Jianxiong;Chen, Xiaolian

作者机构：

关键词： Apoptosis;Oxidative stress;Cell model;IPEC-J2;Weaning

期刊名称：CYTOTECHNOLOGY （ 影响因子：2.058； 五年影响因子：2.362 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We previously demonstrated that intestinal epithelial cell apoptosis in weaned piglets is much more serious than that observed in sucking piglets and is related to oxidative stress during weaning. It is difficult to study the apoptosis mechanisms only using in vivo methods because of the limit of existing research technology. An in vitro cellular system is required for piglet intestinal epithelial cell apoptosis research. In this study, a non-tumorigenic epithelial cell line, IPEC-J2 cells, was employed as a cell model. Hydrogen peroxide and xanthine/xanthine oxidase (X/XO) were both used and compared for apoptosis modeling. The concentrations of hydrogen peroxide and XO were selected and verified using cell viability analysis, the comet assay and flow cytometry. Intracellular ROS were measured using fluorescent probes. Additionally, the expression levels of the apoptosis-related genes Fas, Bcl-2, P53, Caspase 3, Caspase 8, and Caspase 9 were analyzed using quantitative RT-PCR. The results indicated the optimal modeling method is a final concentration of 0.5 mM H2O2 incubated with IPEC-J2 cells for 1 h at 37 A degrees C in 5 % CO2 for hydrogen peroxide-induced apoptosis modeling, and a final concentration of 250 mu M X/50 U/L XO incubated with IPEC-J2 cells for 6 h at 37 A degrees C in 5 % CO2 for X/XO-induced apoptosis modeling. For the apoptotic pathway, the X/XO modeling method is more similar to 21 days weaning piglets. Therefore, we suggest that X/XO modeling with IPEC-J2 cells be used as an in vitro cell culture model for weaning piglet intestinal epithelial cell apoptosis.

分类号： Q2`Q1

  • 相关文献

[1]Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells. Cai, Xuan,Chen, Xiaolian,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Cai, Xuan,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Chen, Xiaolian. 2013

[2]Over-expression of miR-146b and its regulatory role in intestinal epithelial cell viability, proliferation, and apoptosis in piglets. Tao, Xin,Liu, Shujie,Men, Xiaoming,Xu, Ziwei. 2017

[3]Oxidative Stress-Mediated Cytotoxicity of Cadmium in Chicken Splenic Lymphocytes. Li, Jin-Long,Tang, Zhao-Xin,Li, Jin-Long,Li, Shu,Xu, Shi-Wen,Li, Hui-Xin,Wang, Xiao-Long. 2010

[4]Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials. Cui, Jianghu,Yang, Yunhua,Zheng, Mingtao,Liu, Yingliang,Xiao, Yong,Lei, Bingfu,Chen, Wei. 2014

[5]Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells. Wang Lin,Liu Gang,Wang Zhen Yong,Lin Shu Qian,He Yuan Long. 2013

[6]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

[7]Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Jia, Rui,Huo, Huan-Huan,Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Yin, Shu-Ting,Han, Cen.

[8]Rhizoma smilacis glabrae protects rats with gentamicin-induced kidney injury from oxidative stress-induced apoptosis by inhibiting caspase-3 activation. Liu, Cuiyan,Kang, Youxi,Yang, Zisheng,Han, Chunyang,Zhou, Xiuhong,Gu, Jingang.

[9]Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-8 from the blunt snout bream (Megalobrama amblycephala) exposed to ammonia. Sun, Shengming,Ge, Xianping,Zhu, Jian,Zhang, Wuxiao,Zhang, Qiong.

[10]Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Guo, Zhi-Xun.

[11]Cytoprotective effect of deferiprone against aluminum chloride-induced oxidative stress and apoptosis in lymphocytes. Zhuang, Cuicui,Zhang, Haiyang,Song, Miao,Han, Yanfei,Li, Yanfei,She, Yue,Zhu, Yanzhu. 2018

[12]Long-term High-fat High-sucrose Diet Promotes Enlarged Islets and beta-Cell Damage by Oxidative Stress in Bama Minipigs. Zhao, Zhan-zhao,Xin, Lei-lei,Xia, Ji-han,Yang, Shu-lin,Li, Kui,Zhao, Zhan-zhao,Chen, Yao-xing. 2015

[13]Protective Effects of Cynaroside Against H2O2-Induced Apoptosis in H9c2 Cardiomyoblasts. Sun, Xiao-bo,Sun, Xiao-bo. 2011

[14]Carbendazim has the potential to induce oxidative stress, apoptosis, immunotoxicity and endocrine disruption during zebrafish larvae development. Jiang, Jinhua. 2015

[15]Pretilachlor has the potential to induce endocrine disruption, oxidative stress, apoptosis and immunotoxicity during zebrafish embryo development. Jiang, Jinhua,Chen, Yanhong,Yu, Ruixian,Zhao, Xueping,Wang, Qiang,Cai, Leiming. 2016

[16]An isoflavonoid-enriched extract from Pueraria lobata (kudzu) root protects human umbilical vein endothelial cells against oxidative stress induced apoptosis. Gao, Ying,Gao, Ying,Wang, Xu,He, Chunnian.

[17]Neuroprotection against hydrogen peroxide-induced toxicity by Dictyophora echinovolvata polysaccharide via inhibiting the mitochondria-dependent apoptotic pathway. Yu, Wen-Xuan,Dong, Xiao-Li,Lin, Chen-Qiang,Lin, Xin-Jian,Yu, Wen-Xuan,Dong, Xiao-Li,Yu, Wen-Xuan,Dong, Xiao-Li,Zhao, Qing.

[18]Growth performance and rumen microorganism differ between segregated weaning lambs and grazing lambs. Ji Shou-kun,Jiang Cheng-gang,Diao Qi-yu,Tu Yan,Zhang Nai-feng,Si Bing-wen,Li Rui. 2016

[19]Transient changes of enzyme activities and expression of stress proteins in the small intestine of piglets after weaning. Tao, Xin,Xu, Ziwei,Men, Xiaoming.

[20]Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R+IPEC-J2 cell through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway. Qi, K. K.,Sun, Y. Q.,Wan, J.,Deng, B.,Men, X. M.,Wu, J.,Xu, Z. W..

作者其他论文 更多>>

微信小程序