Characterization and pathogenicity of a novel mammalian orthoreovirus from wild short-nosed fruit bats
文献类型: 外文期刊
第一作者: Li, Zhijie
作者: Li, Zhijie;Liu, Dafei;Ran, Xuhua;Liu, Chunguo;Guo, Dongchun;Hu, Xiaoliang;Tian, Jin;Zhang, Xiaozhan;Shao, Yuhao;Liu, Shengwang;Qu, Liandong
作者机构:
关键词: Mammalian orthoreoviruses;Genome sequencing;Pathogenicity;Reassortment;Bat
期刊名称:INFECTION GENETICS AND EVOLUTION ( 影响因子:3.342; 五年影响因子:3.188 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Mammalian orthoreoviruses (MRVs) have a wide range of geographic distribution and have been isolated from humans and various animals. This study describes the isolation, molecular characterization and analysis of pathogenicity of MRV variant B/03 from wild short-nosed fruit bats. Negative stain electron microscopy illustrated that the B/03 strain is a non-enveloped icosahedral virus with a diameter of 70 nm. Sodiumdodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) migration patterns showed that the B/03 viral genome contains 10 segments in a 3:3:4 arrangement. The isolate belongs to MRV serotype 1 based on S1 gene nucleotide sequence data. BALB/c mice experimentally infected with B/03 virus by intranasal inoculation developed severe respiratory distress with tissue damage and inflammation. Lastly, B/03 virus has an increased transmission risk between bats and humans or animals. (C) 2016 Elsevier B.V. All rights reserved.
分类号: R1
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