Detection and quantification of airborne inoculum of Blumeria graminis f. sp. tritici using quantitative PCR

文献类型： 外文期刊

第一作者： Cao, Xueren

作者： Cao, Xueren;Yao, Dongming;Zhou, Yilin;Fan, Jieru;Duan, Xiayu;Yao, Dongming;Ding, Kejian;West, Jonathan S.;Xu, Xiangming;Luo, Yong

作者机构：

关键词： Blumeria graminis f. sp tritici;Wheat powdery mildew;Real-time PCR;Airborne conidia;Spore trap

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, a real-time PCR assay was established to quantify airborne inoculum of Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew. Burkard 7-day recording spore traps were used to collect Bgt spores in air in Langfang City, Hebei Province, China. A linear relationship between number of spores counted with a compound microscope and those determined with the real-time PCR assay was obtained in artificially inoculated spore trap tape sections. The dynamics of airborne conidia concentrations of Bgt were similar. There were significant correlations (P < 0.01) between spore concentrations by using a compound microscope and the real-time PCR assay in three growing seasons. The results demonstrated a potential method to quantitatively determine spore inoculum potential in wheat fields by using a real-time PCR assay.

分类号： S432.1

  • 相关文献

[1]Dynamics in concentrations of Blumeria graminis f. sp tritici conidia and its relationship to local weather conditions and disease index in wheat. Cao, Xueren,Duan, Xiayu,Zhou, Yilin,Luo, Yong. 2012

[2]Detection of Wheat Powdery Mildew with Hyperspectral Remote Sensing Technology and Aerial Digital Image. Cheng Dengfa. 2008

[3]Effects of Powdery Mildew on 1000-Kernel Weight, Crude Protein Content and Yield of Winter Wheat in Three Consecutive Growing Seasons. Yao Dong-ming,Duan Xia-yu,Fan Jie-ru,Zhou Yi-lin,Cao Xue-ren,Yao Dong-ming,Liu Wei,Ding Ke-jian. 2014

[4]The Effect of Wheat Mixtures on the Powdery Mildew Disease and Some Yield Components. Jia Shao-feng,Wang Xiu-na,Duan Xia-yu,Zhou Yi-lin,Jia Shao-feng,Wang Zong-hua,Lu Guo-dong. 2012

[5]Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp tritici during Conidiation. Zeng, Fan-Song,Yang, Li-Jun,Yu, Da-Zhao,Zeng, Fan-Song,Xue, Min-Feng,Zhang, Xue-Jiang,Gong, Shuang-Jun,Yang, Li-Jun,Shi, Wen-Qi,Yu, Da-Zhao,Zeng, Fan-Song,Xue, Min-Feng,Zhang, Xue-Jiang,Gong, Shuang-Jun,Yang, Li-Jun,Shi, Wen-Qi,Yu, Da-Zhao,Menardo, Fabrizio. 2017

[6]Genetic analysis of a novel broad-spectrum powdery mildew resistance gene from the wheat-Agropyron cristatum introgression line Pubing 74. Lu, Yuqing,Yao, Miaomiao,Zhang, Jinpeng,Song, Liqiang,Liu, Weihua,Yang, Xinming,Li, Xiuquan,Li, Lihui.

[7]Control of plant diseases by extracts of Inula viscosa. Wang, W,Ben-Daniel, BH,Cohen, Y. 2004

[8]Molecular mapping of quantitative trait loci for adult-plant resistance to powdery mildew in Italian wheat cultivar Libellula. M. A. Asad,B. Bai,C. X. Lan,J. Yan,X. C. Xia,Y. Zhang,Z. H. He. 2012

[9]Pyramiding adult-plant powdery mildew resistance QTLs in bread wheat. Bai, B.,He, Z. H.,Asad, M. A.,Lan, C. X.,Zhang, Y.,Xia, X. C.,Yan, J.,Chen, X. M.,Bai, B.,Wang, C. S.,He, Z. H.,Bai, B.. 2012

[10]Population Genetic Analysis of Blumeria graminis f. sp tritici in Qinghai Province, China. Duan Xia-yu,Zhou Yi-lin,Guo Qing-yun,Yao Qiang,Cao Shi-qin. 2014

[11]Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2. Ma, Pengtao,Xu, Hongxing,Luo, Qiaoling,Qie, Yanmin,Xu, Yunfeng,An, Diaoguo,Ma, Pengtao,Zhou, Yilin,Han, Haiming,Li, Lihui.

[12]Assessment of partial resistance to powdery mildew in Chinese wheat varieties. Yu, DZ,Yang, XJ,Yang, LJ,Jeger, MJ,Brown, JKM. 2001

[13]Virulence and Diversity of Blumeria graminis f. sp. tritici Populations in China. Zeng Fan-song,Yang Li-jun,Gong Shuang-jun,Zhang Xue-jiang,Wang Hua,Xiang Li-bo,Xue Min-Feng,Yu Da-zhao,Zeng Fan-song,Yu Da-zhao. 2014

[14]An approach for long-term preservation of Blumeria graminis f. sp tritici. Gong, Shuangjun,Yang, Lijun,Xiang, Libo,Shi, Wenqi,Xue, Minfeng,Zeng, Fansong,Zhang, Xuejiang,Wang, Hua,Yu, Dazhao.

[15]Molecular cloning of cDNA of gonadotropin-releasing hormones in the Chinese sturgeon (Acipenser sinensis) and the effect of 17 beta-estradiol on gene expression. Yue, Huamei,Ye, Huan,Chen, Xihua,Li, Chuangju,Cao, Hong,Yue, Huamei,Li, Chuangju. 2013

[16]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[17]CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii. Fei, Xiaowen,Yu, Junmei,Li, Yajun,Deng, Xiaodong. 2017

[18]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[19]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

[20]Preliminary serological evidence for Schmallenberg virus infection in China. Zhai, Shao-Lun,Lv, Dian-Hong,Wen, Xiao-Hui,Zhu, Xue-Liang,Yang, Yan-Qiu,Chen, Qin-Ling,Wei, Wen-Kang. 2018

作者其他论文 更多>>

微信小程序