Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei

文献类型： 外文期刊

第一作者： Fu, Bo

作者： Fu, Bo;Liu, Di;Ma, Hong;Guo, Zhen-Hua;Wang, Liang;Li, Zhong-Qiu;Peng, Fu-Gang;Liu, Di;Bai, Jing

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关键词： cloning efficiency;nuclear factors;porcine oocytes;reprogram;somatic cell nuclear transfer;tetraploid

期刊名称：CELL BIOLOGY INTERNATIONAL （ 影响因子：3.612； 五年影响因子：3.055 ）

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收录情况： SCI

摘要： Cloning efficiency in mammalian systems remains low because reprogramming of donor cells is frequently incomplete. Nuclear factors in the oocyte are removed by enucleation, and this removal may adversely affect reprogramming efficiency. Here, we investigated the role of porcine oocyte nuclear factors during reprogramming. We introduced somatic cell nuclei into intact MII oocytes to establish tetraploid somatic cell nuclear transfer (SCNT) embryos containing both somatic nuclei and oocyte nuclei. We then examined the influence of the oocyte nucleus on tetraploid SCNT embryo development by assessing characteristics including pronucleus formation, cleavage rate, and blastocyst formation. Overall, tetraploid SCNT embryos have a higher developmental competence than do standard diploid SCNT embryos. Therefore, we have established an embryonic model in which a fetal fibroblast nucleus and an oocyte metaphase II plate coexist. Tetraploid SCNT represents a new research platform that is potentially useful for examining interactions between donor nuclei and oocyte nuclei. This platform should facilitate further understanding of the roles played by nuclear factors during reprogramming.

分类号： Q2

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