A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide

文献类型： 外文期刊

第一作者： Chen, Lei

作者： Chen, Lei;Yan, Jie;Sun, Weiping;Zhang, Yan;Feng, Lijun;Sui, Chao;Qi, Jing;Du, Yijun

作者机构：

关键词： Innate immunity;Zebrafish intelectin;LPS/PGN;Bacterial agglutination

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Intelectins are glycan-binding lectins found in various species including cephalochordates, urochordates, fish, amphibians and mammals. But their detailed functions are not well studied in zebrafish which is a good model to study native immunity. In this study, we cloned a zebrafish intelectin ortholog, zebrafish intelectin 2 (zITLN2), which contains a conserved fibrinogen-related domain (FReD) in the N-terminus and the unique intelectin domain in the C-terminus. We examined the tissue distribution of zITLN2 in adult zebrafish and found that zITLN2 was expressed in various organs with the highest level in intestine. Like amphioxus intelectins, zITLN2 expression was upregulated in adult zebrafish infected with Staphylococcus aureus with the highest expression level at 12 h after challenge. Recombinant zITLN2 protein expressed in E. coli was able to agglutinate both Gram-negative and Gram-positive bacteria to similar degrees in a calcium-dependent manner. Furthermore, recombinant zITLN2 bound lipopolysaccharide (LPS) and peptidoglycan (PGN) comparably.

分类号： S9

  • 相关文献

[1]Peptidoglycan Recognition Protein S2 From Silkworm Integument: Characterization, Microbe-Induced Expression, and Involvement in the Immune-Deficiency Pathway. Yang, Jie,Wang, Xiaonan,Wu, Jinmei,Tang, Shunming,Shen, Zhongyuan,Wu, Jinmei. 2015

[2]Immunity to Avian Leukosis Virus: Where Are We Now and What Should We Do?. Feng, Min,Zhang, Xiquan,Feng, Min,Zhang, Xiquan,Feng, Min,Zhang, Xiquan. 2016

[3]Effects of three strains of intestinal autochthonous bacteria and their extracellular products on the immune response and disease resistance of common carp, Cyprinus carpio. Chi, Cheng,Jiang, Bing,Yu, Xiao-Bo,Liu, Tian-Qiang,Wang, Gao-Xue,Xia, Lei. 2014

[4]Characterization of the Mx and hepcidin genes in Epinephelus akaara asymptomatic carriers of the nervous necrosis virus. Mao, Ming-Guang,Jiang, Jie-Lan,Wang, Ke-Jian,Lei, Ji-Lin,Lei, Ji-Lin,Mao, Ming-Guang,Lei, Ji-Lin,Peralvarez-Marin, Alex. 2013

[5]The innate immunity of guinea pigs against highly pathogenic avian influenza virus infection. Zhang, Kun,Xu, Wei wei,Zhang, Zhaowei,Liu, Jing,Sun, Weiyang,Sang, Xiaoyu,Ren, Zhiguang,Yu, Zhijun,Li, Yuanguo,Feng, Na,Wang, Tiecheng,Wang, Hualei,Yang, Songtao,Zhao, Yongkun,Gao, Yuwei,Xia, Xianzhu,Zhang, Kun,Li, Jing,Liu, WenJun,Sun, Lijuan,Zhang, Xuemei,Jiao, Peirong,Liao, Ming,Wilker, Peter R.,Chen, Hualan. 2017

[6]Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity. Yang, Lishi,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan,Qiu, Lihua,Huang, Jianhua,Jiang, Shigui,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan. 2013

[7]Molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (GILT) gene from pearl oyster Pinctada fucata. Zhang, Dianchang,Pan, Dequan,Cui, Shuge,Su, Tianfeng,Qiu, Lihua,Zhu, Caiyan,Jiang, Shigui,Pan, Dequan,Cui, Shuge. 2010

[8]Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis). Sha, Zhen-Xia,Wang, Qi-Long,Chen, Song-Lin,Liu, Yang. 2012

[9]cDNA cloning and mRNA expression of a tandem-repeat galectin (PoGal2) from the pearl oyster, Pinctada fucata. Zhang, D. C.,Hu, Y. T.,Guo, H. Y.,Cui, S. G.,Su, T. F.,Jiang, S. G.,Hu, Y. T.,Guo, H. Y.,Jiang, S. G.. 2011

[10]Two myeloid differentiation factor 88 (MyD88) isoforms identified in ducks. Cheng, Yuqiang,Wang, Hengan,Yan, Yaxian,Sun, Jianhe,Ding, Chan.

[11]Transcript Profiling of Toll-Like Receptor mRNAs in Selected Tissues of Mink (Neovison vison). Tong, Mingwei,Yi, Li,Cheng, Yuening,Zhang, Miao,Cao, Zhigang,Wang, Jianke,Zhao, Hang,Lin, Peng,Cheng, Shipeng,Yang, Yong.

[12]A multidomain galectin involved in innate immune response of pearl oyster Pinctada fucata. Zhang, Dianchang,Jiang, Shigui,Hu, Yuting,Cui, Shuge,Guo, Huayang,Wu, Kaichang,Li, Youning,Su, Tianfeng,Hu, Yuting,Guo, Huayang,Cui, Shuge.

[13]Metabolic, immune responses in prawn (Penaeus monodon) exposed to ambient ammonia. Yang, Lishi,Yang, Qibin,Jiang, Shigui,Li, Yong,Zhou, Falin,Li, Tao,Huang, Jianhua.

[14]Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense. Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Hou, Libo,Wang, Yinghui,Gu, Wei,Meng, Qingguo,Wang, Wen,Xiu, Yunji,Liu, Xiaoqian.

[15]The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN-beta signaling pathway. Li, Dan,Yang, Wenping,Yang, Fan,Liu, Huanan,Zhu, Zixiang,Lian, Kaiqi,Liu, Xiangtao,Zheng, Haixue,Lei, Caoqi,Li, Shu,Shu, Hongbing.

[16]A secreted cystatin from the tick Haemaphysalis longicornis and its distinct expression patterns in relation to innate immunity. Zhou, Jinlin,Ueda, Mami,Umemiya, Rika,Battsetseg, Badgar,Boldbaatar, Damdinsuren,Xuan, Xuenan,Fujisaki, Kozo.

[17]Glycyrrhetinic acid extracted from Glycyrrhiza uralensis Fisch induces the expression of Toll-like receptor 4 in Ana-1 murine macrophages. Li, Li,Miao, Jian-Hua,Xu, Yong-Li,Peng, Li-Na,Qiu, Ya-Feng,Gao, Xiao-Qi,Shi, Zi-Xue,Shao, Dong-Hua,Wei, Jian-Chao,Ma, Zhi-Yong,Zhou, Ying. 2011

[18]Differential and spatial regulation of the prophenoloxidase (proPO) and proPO-activating enzyme in cuticular melanization and innate immunity in Bombyx mori pupae. Zou, Feng Ming,Lee, Kwang Sik,Kim, Bo Yeon,Jin, Byung Rae,Zou, Feng Ming,Kim, Hong Ja,Gui, Zhong Zheng,Zhang, Guo Zheng,Guo, Xijie. 2015

[19]Innate Sensing of Influenza A Virus Hemagglutinin Glycoproteins by the Host Endoplasmic Reticulum (ER) Stress Pathway Triggers a Potent Antiviral Response via ER-Associated Protein Degradation. Wang, Bin,Zheng, Yong-Hui,Frabutt, Dylan A.,Riaz, Sana,Schwartz, Richard C.,Zheng, Yong-Hui. 2018

[20]Molecular cloning of Peking duck Toll-like receptor 3 (duTLR3) gene and its responses to reovirus infection. Song, Kaijie,Li, Chuanfeng,Chen, Zongyan,Ding, Chan,Liu, Guangqing,Zhang, Miaotao,Song, Kaijie. 2015

作者其他论文 更多>>

微信小程序