Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus

文献类型: 外文期刊

第一作者: Luo, Jun

作者: Luo, Jun;Shi, Hehe;Niu, Xuefeng;Long, Teng;Zhao, Jing;Tian, Qin;Wang, Yifei;Chen, Hao;Guo, Xiaofeng;Luo, Jun;Shi, Hehe;Niu, Xuefeng;Long, Teng;Zhao, Jing;Tian, Qin;Wang, Yifei;Chen, Hao;Guo, Xiaofeng;Tan, Yeping

作者机构:

关键词: Rabies virus;Canine parvovirus;VP2;Vaccine;Vector;HEP-Flury

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN:

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收录情况: SCI

摘要: Both rabies virus (RABV) and canine parvovirus (CPV) cause lethal diseases in dogs. In this study, both high egg passage Flury (HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein (G) gene were used to express the CPV virion protein 2 (VP2) gene, and were designated rHEP-VP2 and, rHEP-dG-VP2 respectively. The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury. Western blotting indicated that G protein and VP2 were expressed in vitro. The expression of VP2 in Crandell feline kidney cells post-infection by rHEP-VP2 and rHEP-dG-VP2 was confirmed by indirect immunofluorescence assay with antibody against VP2. Immunogenicity of recombinant rabies viruses was tested in Kunming mice. Both rHEP-VP2 and rHEP-dG-VP2 induced high levels of rabies antibody compared with HEP-Flury. Mice immunized with rHEP-VP2 and rHEP-dG-VP2 both had a high level of antibodies against VP2, which can protect against CPV infection. A challenge experiment indicated that more than 80% mice immunized with recombinant RABVs survived after infection of challenge virus standard 24 (CVS-24). Together, this study showed that recombinant RABVs expressing VP2 induced protective immune responses to RABV and CPV. Therefore, rHEP-VP2 and rHEP-dG-VP2 might be potential combined vaccines for RABV and CPV. (C) 2016 Elsevier Ltd. All rights reserved.

分类号: R392

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