Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse Transcription-Loop-Mediated Isothermal Amplification Methods

文献类型: 外文期刊

第一作者: Bao, Hongmei

作者: Bao, Hongmei;Feng, Xiaoxiao;Ma, Yong;Shi, Jianzhong;Zhao, Yuhui;Gu, Linlin;Wang, Xiurong;Chen, Hualan

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期刊名称:JOURNAL OF CLINICAL MICROBIOLOGY ( 影响因子:5.948; 五年影响因子:5.345 )

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收录情况: SCI

摘要: We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription-loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

分类号: R37

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