An indirect ELISA for detection of Theileria spp. antibodies using a recombinant protein (rTlSP) from Theileria luwenshuni

文献类型： 外文期刊

第一作者： He, Haining

作者： He, Haining;Li, Youquan;Liu, Junlong;Liu, Zhijie;Yang, Jifei;Liu, Aihong;Chen, Ze;Ren, Qiaoyun;Guan, Guiquan;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Yin, Hong

作者机构：

关键词： Theileriosis;Indirect ELISA;rTISP

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. Here, an enzyme-linked immunosorbent assay (ELISA) was developed based on Theileria luwenshuni recombinant surface protein (rTISP) and was used in the standardization and validation of an ELISA for the detection of circulating antibodies against ovine and caprine theileriosis. A total of 233 sera samples were used for the calculation of the cut-off value which served as a threshold between the positive and the negative sera. When the positive threshold was chosen as 19% of the specific mean antibody rate, the specificity was 97.9%, and the sensitivity was 97.1%. There was a cross reaction with sera against Theileria uilenbergi and Theileria ovis, and no cross-reaction with sera against Babesia spp. in the ELISA and Western blotting. Two hundred forty samples collected from sheep in Gansu province were detected with blood smears and ELISA, respectively. The results showed that the positive rate of Theileria infection in Gansu province were 63.75% with rTISP-ELISA, and 46.67% with blood smears, respectively. Our test proved that the rTISP ELISA is suitable to diagnose Theileria infection and could be used in serological surveys to map out the prevalence of ovine and caprine theileriosis. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q958.9

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