Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy
文献类型: 外文期刊
第一作者: Liang, Gang
作者: Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Liu, Xinhui
作者机构:
关键词: Aptamer;Biosensor;Ethanolamine;Electrochemical impedance spectroscopy
期刊名称:ANALYTICA CHIMICA ACTA ( 影响因子:6.558; 五年影响因子:6.228 )
ISSN:
年卷期:
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收录情况: SCI
摘要: A label-free sensing assay for ethanolamine (EA) detection based on G-quadruplex-EA binding interaction is presented by using G-rich aptamer DNA (Ap-DNA) and electrochemical impedance spectroscopy (EIS). The presence of K+ induces the Ap-DNA to form a K+-stabilized G-quadruplex structure which provides binding sites for EA. The sensing mechanism was further confirmed by circular dichroism (CD) spectroscopy and EIS measurement. As a result, the charge transfer resistance (RCT) is strongly increased as demonstrated by using the ferro/ferricyanide ([Fe(CN)(6)](3-/4-)) as a redox probe. Under the optimized conditions, a linear relationship between DRCT and EA concentration was obtained over the range of 0.16 nM and 16 nM EA, with a detection limit of 0.08 nM. Interference by other selected chemicals with similar structure was negligible. Analytical results of EA spiked into tap water and serum by the sensor suggested the assay could be successfully applied to real sample analysis. With the advantages of high sensitivity, selectivity and simple sensor construction, this method is potentially suitable for the on-site monitoring of EA contamination. (C) 2016 Elsevier B.V. All rights reserved.
分类号: O65
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