Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy
文献类型: 外文期刊
第一作者: Liang, Gang
作者: Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Man, Yan;Jin, Xinxin;Pan, Ligang;Liang, Gang;Liu, Xinhui
作者机构:
关键词: Aptamer;Biosensor;Ethanolamine;Electrochemical impedance spectroscopy
期刊名称:ANALYTICA CHIMICA ACTA ( 影响因子:6.558; 五年影响因子:6.228 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A label-free sensing assay for ethanolamine (EA) detection based on G-quadruplex-EA binding interaction is presented by using G-rich aptamer DNA (Ap-DNA) and electrochemical impedance spectroscopy (EIS). The presence of K+ induces the Ap-DNA to form a K+-stabilized G-quadruplex structure which provides binding sites for EA. The sensing mechanism was further confirmed by circular dichroism (CD) spectroscopy and EIS measurement. As a result, the charge transfer resistance (RCT) is strongly increased as demonstrated by using the ferro/ferricyanide ([Fe(CN)(6)](3-/4-)) as a redox probe. Under the optimized conditions, a linear relationship between DRCT and EA concentration was obtained over the range of 0.16 nM and 16 nM EA, with a detection limit of 0.08 nM. Interference by other selected chemicals with similar structure was negligible. Analytical results of EA spiked into tap water and serum by the sensor suggested the assay could be successfully applied to real sample analysis. With the advantages of high sensitivity, selectivity and simple sensor construction, this method is potentially suitable for the on-site monitoring of EA contamination. (C) 2016 Elsevier B.V. All rights reserved.
分类号: O65
- 相关文献
作者其他论文 更多>>
-
Effect of Multiyear Biodegradable Plastic Mulch on Soil Microbial Community, Assembly, and Functioning
作者:Liu, Xiaowei;Wen, Zongyu;Wen, Zongyu;Dong, Wentao;Ren, Huiqing;Liang, Gang;Gong, Wenwen;Zhou, Wei
关键词:biodegradable plastics; soil microorganisms; microbial diversity; ecological functions; nutrient cycling
-
Residue regulation and dissipation pattern of five typical pesticides on spinach in cool climate region
作者:Song, Le;Li, An;Yang, Xusheng;Yang, Zixi;Zhao, Yueyuan;Wei, Yufan;Liu, Jiahao;Liang, Gang;Pan, Canping
关键词:Cool open field; Spinach; Pesticide residue; Cumulative exposure; Dietary intake risk assessment
-
CRISPR-Cas12a/Aurora Deoxyribozyme Cascade: A Label-Free Ultrasensitive Platform for Rapid Salmonella Detection
作者:Shi, Cong;Tan, Huimin;Yu, Zhou;Li, Weilin;Man, Yan;Zhang, Qinghai;Shi, Cong;Tan, Huimin;Yu, Zhou;Li, Weilin;Man, Yan
关键词:nucleic acid detection; fluorescence sensing; food safety; DNAzyme; pathogen identification
-
Indirect determination of environmental polylactic acid microplastic residues using a single-atom nickel composite electrode
作者:Pei, Yuexin;Pei, Xinlong;Shang, Ruichao;Wang, Zehui;Li, Long;Zhu, Hong;Liang, Gang;Gong, Wenwen;Yue, Ying
关键词:Environmental monitoring; Mulch contamination; Polylactic acid microplastics; Single-atom Ni; Electrochemical detection
-
Magnetic Multi-Walled Carbon Nanotubes Nanomaterials as Magnetic Solid Phase Extraction Adsorbents for Simultaneous Determination of Multiple Mycotoxins in Agricultural Products by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
作者:Ma, Shuai;Liang, Gang;Man, Yan;Jin, Xin-Xin;Li, An
关键词:Mycotoxins; Magnetic multi-walled carbon nanotubes; Magnetic solid-phase extraction; Ultra-high performance liquid chromatography-tandem mass spectrometry
-
Identification of Putative Quantitative Trait Loci for Improved Seed Oil Quality in Peanuts
作者:Hu, Pengju;Zhang, Jianan;Song, Yahui;Zhao, Xing;Jin, Xinxin;Su, Qiao;Yang, Yongqing;Wang, Jin
关键词:peanut; oil quality; fatty acids; QTL; epistatic effects
-
CuInS2/Red Phosphorus Nanosheet Interleaved Heterostructures with Improved Interfacial Charge Transfer for Photoelectrochemical Aptasensing
作者:Li, Yunpeng;Adili, Guliqire;Ma, Yuhua;Liang, Gang;Liu, Jianbo;Ma, Yuhua
关键词: