Multilocus sequence analysis of the genus Kurthia, and a description of Kurthia populi sp nov.

文献类型： 外文期刊

第一作者： Fang, Wei

作者： Fang, Wei;Guo, Min-wei;Xue, Han;Wang, Lai-fa;Tian, Guo-zhong;Piao, Chun-gen;Li, Yong;Ruan, Zhi-yong

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期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

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收录情况： SCI

摘要： Four novel bacterial strains belonging to the genus Kurthia were isolated from the surface of a weevil of the family Curculionidae (strain 10y-14(T)), and from bark samples of hybrid poplar, Populus x euramericana (strains 6-3, 2-5 and 06C10-3-14), in Puyang, Henan Province, China. Phylogenetic analyses of the 16S rRNA gene and multilocus sequence analysis (MLSA) data showed that the four strains form a distinct cluster in the genus Kurthia, indicating that they all belong to a single taxon within the genus. DNA-DNA hybridization levels between strain 10y-4(T) and Kurthia huakuii LAM0618(T) and Kurthia massiliensis DSM 24639(T) were 58.31 and 53.92 %, respectively. This indicates that the four novel strains represent a species distinct from these two closely related species. The DNA G+C content of the novel strains was 42.1-42.6 %. The major fatty acids were iso-C-15 : 0 and anteiso-C-15 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and unidentified aminophospholipids. The predominant menaquinones were MK-7 (90 %) and MK-6 (10 %). The major cell-wall amino acids were lysine, alanine, glutamic acid and glycine. On the basis of the MLSA and 16S rRNA gene sequence phylogenetic analyses, DNA-DNA reassociation values, DNA base composition, and biochemical and phenotypic characteristics, the four strains are considered to represent a novel species within the genus Kurthia, for which the name Kurthia populi sp. nov. is proposed. The type strain is 10y-14(T) (=CFCC 11600(T)=KCTC 33522(T)).

分类号： Q

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