Flavobacterium endophyticum sp nov., a nifH gene-harbouring endophytic bacterium isolated from maize root

文献类型: 外文期刊

第一作者: Gao, Jun-lian

作者: Gao, Jun-lian;Wang, Xu-ming;Li, Ji-wei;Wu, Qin-yu;Lv, Fan-yang;Yuan, Mei;Sun, Jian-guang;Li, Ji-wei;Wu, Qin-yu

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期刊名称:INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ( 影响因子:2.747; 五年影响因子:2.889 )

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收录情况: SCI

摘要: A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain 522(T), was isolated from surface-sterilized root tissue of maize planted in Fangshan District of Beijing, China. A polyphasic taxonomic study was performed on the new isolate. On the basis of 16S rRNA gene sequence similarity studies, this isolate belonged to the genus Flavobacterium and showed less than 93.9 % similarity to the type strains of all recognized species of the genus Flavobacterium. The predominant respiratory quinone was menaquinone-6 and the polar lipid profile was composed of the major lipids phosphatidylethanolamine, phosphatidylserine and two unidentified amino lipids. The major fatty acids were C-15 : 0, iso-C-15 : 0, iso-C-15 : 1 G and iso-C-16 : 0. The G+C content of the DNA was 37.7 mol%. The results of physiological and biochemical tests and the differences in fatty acid profiles allowed the clear phenotypic differentiation of strain 522(T) from closely related species of the genus Flavobacterium. Strain 522(T) therefore represents a novel species within the genus Flavobacterium, for which the name Flavobacterium endophyticum sp. nov. is proposed. The type strain is 522(T) (=ACCC 19708(T)=DSM 29537(T)).

分类号: Q

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