The effects of gibberellins and mepiquat chloride on nitrogenase activity in Bradyrhizobium japonicum

文献类型： 外文期刊

第一作者： Chen, Wenhao

作者： Chen, Wenhao;Zheng, Dianfeng;Feng, Naijie;Liu, Tao;Liu, Yang;Gong, Shen;Cui, Hongqiu;Cui, Hongqiu;Xiang, Hongtao

作者机构：

关键词： Rhizobia;Plant growth regulators;Two-dimensional electrophoresis;Nitrogenase activity;Nodulation

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Application of plant growth regulators (PGRs) to soybean plants is known to induce changes in nitrogenase activity in root nodules, and this led us to hypothesize that PGRs would affect nitrogenase activity in free-living rhizobia cultures. Little is known about the molecular basis of the effects of PGRs on nitrogenase activity in free-living rhizobia cultures. Therefore, a comparative study was conducted on the effects of gibberellins (GA(3)) and mepiquat chloride (PIX), which regulate plant growth, on the nitrogenase activity of the nitrogen-fixing bacterium Bradyrhizobium japonicum. Fix and nif gene regulation and protein expression in free-living cultures of B. japonicum were investigated using real-time PCR and two-dimensional electrophoresis after treatment with GA(3) or PIX. GA(3) treatment decreased nitrogenase activity and the relative expression of nifA, nifH, and fixA genes, but these effects were reversed by PIX treatment. As expected, several proteins involved in nitrogenase synthesis were down-regulated in the GA(3)-treated group. Conversely, several proteins involved in nitrogenase synthesis were up-regulated in the PIX-treated group, including bifunctional ornithine acetyltransferase/N-acetylglutamate synthase, transaldolase, ubiquinol-cytochrome C reductase iron-sulfur subunit, electron transfer flavoprotein subunit beta, and acyl-CoA dehydrogenase. Two-pot experiments were conducted to evaluate the effects of GA(3) and PIX on nodulation and nitrogenase activity in Rhizobium-treated legumes. Interestingly, GA(3) treatment increased nodulation and depressed nitrogenase activity, but PIX treatment decreased nodulation and enhanced nitrogenase activity. Our data show that the nif and fix genes, as well as several proteins involved in nitrogenase synthesis, are up-regulated by PIX and down-regulated by GA(3), respectively, in B. japonicum.

分类号： Q94

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