A simple and efficient transformation system for the edible mushroom Pleurotus eryngii

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Wang, Rong;Mao, Wen Jun;Gong, Ming;Gao, Ying Nv;Tang, Li Hua;Li, Yan;Zhou, Chen Li;Bao, Da Peng;Yang, Rui Fang

作者机构：

关键词： Agrobacterium tumefaciens-mediated transformation;Glufosinate-ammonium resistance marker;Hsp70 promoter

期刊名称：MYCOSCIENCE （ 影响因子：1.223； 五年影响因子：1.266 ）

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收录情况： SCI

摘要： Although Pleurotus eryngii is a highly-valued mushroom, the tools for undertaking molecular biological research are lacking. We have now developed an improved, more efficient ATMT (Agrobacterium tumefaciens-mediated transformation) system for generating P. eryngii transformants. Successful generation of transformants required that nutrient-limited fungal mycelium be used as the receptor material. The transformants remained genetically stable and produced normal clamp connections in mating tests. The procedures developed here provide an effective and simple means for gene function studies and directed breeding in P. eryngii essential for realizing the full biotechnological potential of this mushroom. (C) 2016 Published by Elsevier B.V. on behalf of The Mycological Society of Japan.

分类号： Q939.5

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