Recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip for equipment-free detection of Cryptosporidium spp. oocysts in dairy cattle feces

文献类型： 外文期刊

第一作者： Wu, Yao-Dong

作者： Wu, Yao-Dong;Zhou, Dong-Hui;Zheng, Wen-Bin;Ma, Jian-Gang;Wang, Meng;Zhu, Xing-Quan;Xu, Min-Jun;Zhang, Long-Xian;Zheng, Wen-Bin;Ma, Jian-Gang;Xu, Min-Jun

作者机构：

关键词： Cryptosporidium spp.;Dairy cattle;N-lauroylsarcosine sodium salt;Recombinase polymerase amplification (RPA);Lateral flow strip

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

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收录情况： SCI

摘要： Cryptosporidium is a widespread protozoan parasite that infects a large number of vertebrate animals, resulting in varying degrees of diarrhea or even death. As dairy cattle feces is an important source of Cryptosporidium spp. infection, development of a handy and accurate detection method via its oocysts in dairy cattle feces would be interesting and necessary. We herein developed a quick detecting method using recombinase polymerase amplification (RPA) combined with lateral flow (LF) strip to detect DNA of Cryptosporidium oocysts in dairy cattle feces. The DNA was released by boiled water with 0.1 % N-lauroylsarcosine sodium salt (LSS). The established method was proven to be of higher sensitivity than normal polymerase chain reaction (PCR) amplification with the lowest detection of 0.5 oocyst per reaction, and specificity with no cross reactivity to other common protozoan species in the intestine of dairy cattle. The diagnostic method established herein is simple, rapid, and cost-effective, and has potential for further development as a diagnostic kit for the diagnosis of cryptosporidiosis of dairy cattle.

分类号： R53

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