Molecular and biochemical characterization of Eimeria tenella hexokinase

文献类型： 外文期刊

第一作者： Sun, Mingfei

作者： Sun, Mingfei;Liao, Shenquan;Wu, Caiyan;Qi, Nanshan;Lv, Minna;Li, Juan;Lin, Xuhui;Zhang, Jianfei;Xie, Mingquan;Cai, Jianping;Sun, Mingfei;Liao, Shenquan;Wu, Caiyan;Qi, Nanshan;Lv, Minna;Li, Juan;Lin, Xuhui;Zhang, Jianfei;Xie, Mingquan;Cai, Jianping;Sun, Mingfei;Liao, Shenquan;Wu, Caiyan;Qi, Nanshan;Lv, Minna;Li, Juan;Lin, Xuhui;Zhang, Jianfei;Xie, Mingquan;Cai, Jianping;Zhang, Longxian;Zhu, Guan

作者机构：

关键词： Eimeria tenella;Hexokinase;Glycolysis;Quercetin;Mangiferin

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Hexokinase (HK) is one of the key enzymes in the glycolytic pathway that catalyzes the phosphorylation of glucose. In the present study, we cloned the HK gene from the coccidian Eimeria tenella (EtHK), expressed EtHK as a His-tagged fusion protein, and characterized its primary biochemical features. Mutagenesis confirmed that residues S159, N216, and D217 are essential or important to the EtHK catalytic activity. EtHK exhibited high affinity for d-glucose (Km = 0.67 to 0.79 mM), but was also able to utilize 2-deoxy-d-glucose (Km = 5.66 mM), d-fructose (Km = 13.76 mM), and d-mannose (Km = 25.41 mM). We also observed that quercetin and mangiferin could inhibit the EtHK enzyme activity (IC50 values = 6.52 and 85.82 mu M, respectively). Among the two inhibitors, mangiferin also inhibited the growth of E. tenella in vitro (MIC50 = 0.12 mu M). These observations suggest that EtHK may be explored as potential drug target, and mangiferin and its analogs may be explored for developing anti-coccidial therapeutics.

分类号： R53

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