Integrin v1 Modulation Affects Subtype B Avian Metapneumovirus Fusion Protein-mediated Cell-Cell Fusion and Virus Infection

文献类型： 外文期刊

第一作者： Yun, Bing-Ling

作者： Yun, Bing-Ling;Guan, Xiao-Lu;Liu, Yong-Zhen;Zhang, Yao;Wang, Yong-Qiang;Qi, Xiao-Le;Cui, Hong-Yu;Liu, Chang-Jun;Zhang, Yan-Ping;Gao, Hong-Lei;Gao, Li;Li, Kai;Gao, Yu-Long;Wang, Xiao-Mei;Wang, Xiao-Mei

作者机构：

关键词： fusion protein;infection;integrin;membrane fusion;viral replication;aMPV;B;fusogenicity

期刊名称：JOURNAL OF BIOLOGICAL CHEMISTRY （ 影响因子：5.157； 五年影响因子：5.041 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Avian metapneumovirus (aMPV) fusion (F) protein mediates virus-cell membrane fusion to initiate viral infection, which requires F protein binding to its receptor(s) on the host cell surface. However, the receptor(s) for aMPV F protein is still not identified. All known subtype B aMPV (aMPV/B) F proteins contain a conserved Arg-Asp-Asp (RDD) motif, suggesting that the aMPV/B F protein may mediate membrane fusion via the binding of RDD to integrin. When blocked with integrin-specific peptides, aMPV/B F protein fusogenicity and viral replication were significantly reduced. Specifically we identified integrin v and/or 1-mediated F protein fusogenicity and viral replication using antibody blocking, small interfering RNAs (siRNAs) knockdown, and overexpression. Additionally, overexpression of integrin v and 1 in aMPV/B non-permissive cells conferred aMPV/B F protein binding and aMPV/B infection. When RDD was altered to RAE (Arg-Ala-Glu), aMPV/B F protein binding and fusogenic activity were profoundly impaired. These results suggest that integrin v1 is a functional receptor for aMPV/B F protein-mediated membrane fusion and virus infection, which will provide new insights on the fusogenic mechanism and pathogenesis of aMPV.

分类号： Q5

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