Identification of a thioredoxin reductase from Babesia microti during mammalian infection

文献类型： 外文期刊

第一作者： Zhao, Shaoruo

作者： Zhao, Shaoruo;Gong, Haiyan;Zhou, Yongzhi;Zhang, Houshuang;Cao, Jie;Zhou, Jinlin;Zhou, Jinlin

作者机构：

关键词： Babesia microti;Thioredoxin reductase;Enzyme activity;Auranofin;Drug target

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Babesia microti is the primary causative agent of human babesiosis worldwide and associated with increased human health risks and the safety of blood supply. The parasite replicates in the host's red blood cells, thus, in order to counteract the oxidative stress and toxic effects, parasites employ a thioredoxin (Trx) system to maintain a redox balance. Since thioredoxin reductase (TrxR) plays a critical role in the system, in this study, we report the cloning, expression, and functional characterization of a novel TrxR from B. microti (BmiTrxR). The complete gene BmiTrxR was obtained by amplifying the 5' and 3' regions of messenger RNA (mRNA) by RACE. The full-length complementary DNA (cDNA) of BmiTrxR was 1766 bp and contained an intact open reading frame with 1662 bp that encoded a polypeptide with 553 amino acids. Molecular weight of the predicted protein was 58.4 kDa with an isoelectric point of 6.95, similar to high molecular weight TrxR. The recombinant protein of BmiTrxR was expressed in a His-fused soluble form in Escherichia coli. The native protein BmiTrxR was identified with the mouse anti-BmiTrxR polyclonal serum by western blotting and IFAT. Moreover, the enzyme showed a disulfide reductase activity using DTNB as substrate and catalyzed the NADPH-dependent reduction of Trx. Auranofin, a known inhibitor of TrxR, completely abrogated the activity of the recombinant enzyme in vitro. These results not only contribute to the understanding of redox pathway in this parasite but also suggest that BmiTrxR could be a potential target for the development of novel strategies to control B. microti thus reducing the incidence of babesiosis.

分类号： R53

  • 相关文献

[1]Molecular characterization of SjBIRP, another apoptosis inhibitor, from Schistosoma japonicum. Dao, Jinwei,Zhu, Lihui,Luo, Rong,Hu, Chao,Wang, Yuqing,Li, Hao,Lu, Ke,Liu, Jinming,Lin, Jiaojiao,Cheng, Guofeng,Dao, Jinwei,Cheng, Guofeng. 2014

[2]Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs. Huang, Jingwei,Xiong, Kang,Zhang, Houshuang,Zhao, Yanzhen,Cao, Jie,Gong, Haiyan,Zhou, Yongzhi,Zhou, Jinlin. 2018

[3]Comparative genomic analysis of aspartic proteases in eight parasitic platyhelminths: Insights into functions and evolution. Wang, Shuai,Wei, Wei,Luo, Xuenong,Cai, Xuepeng,Wang, Sen,Hu, Songnian.

[4]Expression of truncated Babesia microti apical membrane protein 1 and rhoptry neck protein 2 and evaluation of their protective efficacy. Wang, Guanbo,Efstratiou, Artemis,Moumouni, Paul Franck Adjou,Liu, Mingming,Jirapattharasate, Charoonluk,Guo, Huanping,Gao, Yang,Suzuki, Hiroshi,Igarashi, Ikuo,Xuan, Xuenan,Cao, Shinuo,Zhou, Mo.

[5]Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA). Cao, Shinuo,Luo, Yuzi,Aboge, Gabriel Oluga,Terkawi, Mohamad Alaa,Masatani, Tatsunori,Suzuki, Hiroshi,Igarashi, Ikuo,Nishikawa, Yoshifumi,Xuan, Xuenan,Luo, Yuzi.

[6]Genome-wide analysis of regulatory proteases sequences identified through bioinformatics data mining in Taenia solium. Yan, Hong-Bin,Lou, Zhong-Zi,Li, Li,Zheng, Yadong,Luo, Xuenong,Hou, Junling,Guo, Aijiang,Jia, Wan-Zhong,Cai, Xuepeng,Yan, Hong-Bin,Brindley, Paul J.,Yan, Hong-Bin,Brindley, Paul J.. 2014

[7]Identification of 2-Cys Peroxiredoxin (BmTPx-2) as Antioxidant Active Molecule from Babesia microti. Hai, Xunan,Zhang, Houshuang,Wang, Zhonghua,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin. 2017

[8]Babesia microti Aldo-keto Reductase-Like Protein Involved in Antioxidant and Anti-parasite Response. Huang, Qiang,Cao, Jie,Zhou, Yongzhi,Huang, Jingwei,Gong, Haiyan,Zhang, Houshuang,Zhou, Jinlin,Zhu, Xing-Quan,Zhu, Xing-Quan,Zhou, Jinlin. 2017

[9]Evaluation on Infectivity of Babesia microti to Domestic Animals and Ticks Outside the Ixodes Genus. Wu, Jiajun,Cao, Jie,Zhou, Yongzhi,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[10]Application of genomics and proteomics in drug target discovery. Zhang, H. M.,Nan, Z. R.,Hui, G. Q.,Liu, X. H.,Sun, Y.,Zhang, H. M.,Nan, Z. R.,Hui, G. Q.,Sun, Y.. 2014

[11]Identification and functional study of a novel 2-cys peroxiredoxin (BmTPx-1) of Babesia microti. Zhang, Houshuang,Wang, Zhonghua,Gong, Haiyan,Cao, Jie,Zhou, Yongzhi,Zhou, Jinlin,Zhou, Jinlin.

[12]A Unique Hexokinase in Cryptosporidium parvum, an Apicomplexan Pathogen Lacking the Krebs Cycle and Oxidative Phosphorylation. Yu, Yonglan,Yu, Yonglan,Zhang, Haili,Guo, Fengguang,Zhu, Guan,Sun, Mingfei.

[13]Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Qinghua Wang,Xue Zhang,Fuguang Li,Yuxia Hou,Xingliang Liu,Xueyan Zhang.

[14]Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by Pseudomonas putida. Jing Yongping,Li Yan,Zhang Yingpeng,Liu Ping,Sun Ming,Jing Yongping,Li Yan,Zhang Yingpeng,Jing Yongping,Liu Zhaohui,Luo, Jiafa. 2017

[15]Response of the enzymes to nitrogen applications in cotton fiber (Gossypium hirsutum L.) and their relationships with fiber strength. Wang YouHua,Feng Ying,Xu NaiYin,Chen BingLin,Ma RongHui,Zhou ZhiGuo,Xu NaiYin. 2009

[16]Physiological and morphological responses induced by alpha-particle radiation on Arabidopsis thaliana embryos. Ren, J.,Liu, L.,Fu, S. L.,Ren, J.,Jin, X. L.,Ding, Z. C.,Liu, L.. 2014

[17]Canonical correspondence analysis of soil heavy metal pollution, microflora and enzyme activities in the Pb-Zn mine tailing dam collapse area of Sidi village, SW China. Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Yang, Rongmei,Tang, Huafeng,Li, Min,Huang, Bingfu,Zhang, Jiayu,Li, Guiwen,Li, Zhongyi,Hu, Qingjing. 2015

[18]Sucrose and citric acid accumulations in melon genotypes with different sugar and acid contents. Tang, Mi,Zhang, Bao-cai,Xie, Jun-jun,Bie, Zhi-long,Wu, Ming-zhu,Yi, Hong-ping,Feng, Jong-xin,Tang, Mi. 2012

[19]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[20]Distinct responses of soil bacterial and fungal communities to changes in fertilization regime and crop rotation. Zhang, Shuiqing,Guo, Doudou,Huang, Shaomin,Ai, Chao,Zhang, Xin,Zhou, Wei. 2018

作者其他论文 更多>>

微信小程序