A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for 5-Formyltetrahydrofolate Detection in Maize Kernels

文献类型： 外文期刊

第一作者： Yue, Huanfang

作者： Yue, Huanfang;Zhang, Wei;Cao, Zhen;Tan, Guiyu;Wang, Baomin;Yue, Huanfang;Liang, Qiuju;Zhang, Chunyi

作者机构：

关键词： 5-Formyltetrahydrofolate;Monoclonal antibody;ELISA;Maize kernels

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Maize is one of the most important staple food crops worldwide. 5-Formyltetrahydrofolate (5-F-THF), the most dominant and stable folate derivative in maize kernels, is important to human health and acts key roles in several metabolic pathways and biological processes. To assist investigating maize germplasm and subsequent breeding of maize varieties containing high levels of 5-F-THF, a cost-effective and high-throughput methodology for 5-F-THF detection is required. In the present study, monoclonal antibodies (mAbs) against 5-F-THF were raised in mice, followed by establishment of an indirect competitive enzyme-linked immunosorbent assay (icELISA). The icELISA showed an IC50 of 35.10 ng/mL and a working range of 7.90-263.89 ng/mL for 5-F-THF. The analytical recovery for 5-F-THF in the maize kernels with icELISA was 66-111 %, and that with liquid chromatography-mass spectrometry (LC-MS) was 57-114 %, indicating a good consistency between these two methods (Slope, 0.92; R (2), 0.98). We conclude that the sensitive and versatile icELISA can be used for high-throughput detection of 5-F-THF in maize kernels.

分类号： TS2

  • 相关文献

[1]Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples. Li WeiHua,Liu Wei,Yin WeiWei,Xu Jing,Liu JinTing,Meng Meng,Xue HuYin,Xi RiMo,Meng Meng,Xue HuYin,Xi RiMo,He FangYang,Wan YuPing,Feng CaiWei,Wang ShanLiang,Lu Xiao. 2011

[2]Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene. Qiao, Bin,Li, Yansong,Meng, Xingyu,Sun, Yu,Hu, Pan,Lu, Shiying,Ren, Honglin,Liu, Zengshan,Zhou, Yu,Meng, Xingyu,Sun, Yu,Zhou, Yu.

[3]Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins. Li, Peiwu,Zhou, Qian,Wang, Ting,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Li, Peiwu,Zhou, Haiyan,Zhang, Wen,Ding, Xiaoxia,Zhang, Zhaowei,Zhang, Qi,Zhou, Qian,Chang, Perng-Kuang. 2016

[4]Production and characterization of monoclonal antibody for class-specific determination of O,O-dimethyl organophosphorus pesticides and effect of heterologous coating antigens on immunoassay sensitivity. Liu, Yan,Lou, Yang,Xu, Dan,Qian, Guoliang,Wu, Runru,Hu, Baishi,Liu, Fengquan,Zhang, Qi.

[5]Preparation and Identification of a Monoclonal Antibody Specific to a Broad Spectrum of O, O-diethyl Organophosphorus Pesticides. Zhang Li-Jie,Pan Jia-Rong. 2010

[6]Preparation of Monoclonal Antibody for Melamine and Development of an Indirect Competitive ELISA for Melamine Detection in Raw Milk, Milk Powder, and Animal Feeds. Zhang, Taichang,Meng, Meng,Xi, Rimo,Yin, Weiwei,Liu, Jinting,Li, Weihua,Liu, Wei,He, Fangyang,Wan, Yuping,Feng, Caiwei,Wang, Shanliang,Lu, Xiao.

[7]Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3. Liu, Zhenxing,Hao, Le,Ma, Yanping,Liang, Zhiling,Ma, Jiangyao,Ke, Hao.

[8]Distribution and variation of fungi and major mycotoxins in pre- and post-nature drying maize in North China Plain. Xing, Fuguo,Liu, Xiao,Wang, Limin,Selvaraj, Jonathan Nimal,Jin, Nuo,Wang, Yan,Zhao, Yueju,Liu, Yang.

[9]Development of Monoclonal Antibody-Based Sensitive Sandwich ELISA for the Detection of Antinutritional Factor Cowpea Trypsin Inhibitor. G. Y. Tan ,T. G. Nan,W. Gao,Q. X. Li,J. J. Cui,B. M. Wang.

[10]Effects of Monoclonal Antibody Against Porcine 40-kDa Adipocyte-Specific Membrane Protein on Endocrine Secretion in Pigs. Zhao Su-mei,Zhang Xi,Duan Gang,Gao Shi-zheng,Hu Hong-mei,Liu Ling-yun. 2009

[11]Development of Sensitive Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay for Renal Toxicity Ingredient Aristolochic acid A in Chinese Herb Medicine. Nan Tie-Gui,He Su-Ping,Tan Gui-Yu,Wang Bao-Min,Li Gang,Huang Lu-Qi. 2010

[12]Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China. Chen, Yu-Han,Chien, Wan-Chu,Sun, Jing-Hua,Wang, Yun-Chi,Chen, Tsung-Chi,Dong, Jiahong,Zheng, Kuanyu,Wu, Kuo,Yeh, Shyi-Dong,Yeh, Shyi-Dong,Chen, Tsung-Chi. 2016

[13]An ultra-sensitive monoclonal antibody-based competitive enzyme immunoassay for aflatoxin M-1 in milk and infant milk products. Guan, Di,Li, Peiwu,Zhang, Wen,Zhang, Daohong,Jiang, Jun,Guan, Di,Li, Peiwu,Zhang, Qi,Zhang, Wen,Zhang, Daohong,Jiang, Jun,Guan, Di,Li, Peiwu,Zhang, Qi. 2011

[14]Development of an antigen-capture ELISA for the detection of equine influenza virus nucleoprotein. Ji, Yuanyuan,Guo, Wei,Zhao, Liping,Li, Hongmei,Lu, Gang,Wang, Zheng,Wang, Guibin,Liu, Cuiyun,Xiang, Wenhua. 2011

[15]Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit. Man, Yan,Liang, Gang,Li, An,Fu, Hailong,Wang, Meng,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Fu, Hailong,Wang, Meng,Pan, Ligang,Man, Yan,Liang, Gang,Li, An,Fu, Hailong,Wang, Meng,Pan, Ligang,Jia, Fuchao. 2017

[16]Simultaneous production of monoclonal antibodies against Bacillus thuringiensis (Bt) Cry1 toxins using a mixture immunization. Dong, Sa,Zhang, Cunzheng,Liu, Yuan,Zhang, Xiao,Xie, Yajing,Zhong, Jianfeng,Xu, Chongxin,Liu, Xianjin,Dong, Sa,Liu, Xianjin. 2017

[17]Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. Yun, Bingling,Li, Delong,Zhu, Haibo,Liu, Wen,Qin, Liting,Liu, Zaisi,Wu, Guan,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei,Gao, Yulong. 2013

[18]An approach for isolating high-molecular-weight glutenin subunit genes using monoclonal antibodies. Wang, HQ,Zhang, XY. 2006

[19]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[20]Eu3+-labeled IgG-based time-resolved fluoroimmunoassay for highly sensitive detection of aflatoxin B-1 in feed. Zhang, Gaiping,Hu, Xiaofei,Yao, Jingjing,Wang, Fangyu,Yin, Mengqi,Sun, Yaning,Hu, Mei,Shi, Qiaoqiao,Zhang, Gaiping. 2018

作者其他论文 更多>>

微信小程序