Comparative proteomic analysis reveals intracellular targets for bacillomycin L to induce Rhizoctonia solani Kuhn hyphal cell death

文献类型: 外文期刊

第一作者: Zhang, Bao

作者: Zhang, Bao;Qin, Yuxuan;Han, Yuzhu;Li, Pinglan;Qin, Yuxuan;Li, Pinglan;Zhang, Bao;Dong, Chunjuan;Shang, Qingmao

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关键词: Bacillomycin L;Iturin;Rhizoctonia solani Kuhn;Proteome;Antibiotics

期刊名称:BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS ( 影响因子:3.036; 五年影响因子:2.969 )

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收录情况: SCI

摘要: Bacillomycin L, a natural iturinic lipopeptide produced by Bacillus amyloliquefaciens, is characterized by strong antifungal activity against a variety of agronomically important filamentous fungi including Rhizoctonia solani Kuhn To further understand its antifungal actions, proteomes were comparatively studied within R. solani hyphal cells treated with or without bacillomycin L The results show that 39 proteins were alternatively expressed within cells in response to this lipopeptide, which are involved in stress response, carbohydrate, amino acid and nucleotide metabolism, cellular component organization, calcium homeostasis, protein degradation, RNA processing, gene transcription, and others, suggesting that, in addition to inducing cell membrane permeabilization, iturin exhibits antibiotic activities by targeting intracellular molecules. Based on these results, a model of action of bacillomycin L against R. solani hyphal cells was proposed. Our study provides new insight into the antibiotic mechanisms of iturins. (C) 2016 Elsevier B.V. All rights reserved.

分类号: Q5

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