Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH

文献类型: 外文期刊

第一作者: Ren, Xiaohua

作者: Ren, Xiaohua;Zhou, Yuqin;Cao, Xiaohan;Du, Xiaogang;Meng, Fengyan;Tan, Yao;Liu, Yacheng;Zeng, Xianyin;Zeng, Yu;Song, TianZeng;Feng, Jing;Chu, Mingxing

作者机构:

关键词: Reproductive axis;Spleen;Immunocastration;GnRH;Immune cytokines;Ram

期刊名称:INTERNATIONAL IMMUNOPHARMACOLOGY ( 影响因子:4.932; 五年影响因子:4.624 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Hypothalamic-pituitary-gonadal (HPG) axis is strongly implicated in the regulation of immune system. The objective was to determine the effects of immunocastration on splenic reproduction- and immunity-related gene expressions, and serum cytokine profiles in rams. Forty rams were randomly allocated into three groups: control (n = 14); surgically castrated (n = 13); or immunized (n = 13) against 100 mu g D-Lys6-GnRH-tandem-dimer peptide conjugated to ovalbumin in Specol adjuvant at 6 months of age (with a booster 2 months later). Blood samples (for hormone and immune cytokine profiles) were collected at 1-month intervals until rams were slaughtered (10 months). Compared to intact controls, anti-GnRH immunization reduced (P < 0.05) serum concentrations of LH, FSH, and testosterone. Reduced testosterone abrogated its inhibitor feedback effect on the synthesis of GnRH in spleen, as evidenced by increased (P < 0.05) protein content and mRNA expressions of GnRH, and simultaneously decreased (P < 0.05) mRNA expressions of androgen receptor in spleen. In parallel with the increased GnRH production in spleen, the mRNA expressions of interleukin (IL)-2, IL-4, IL-6 and tumor necrosis factor alpha (TNF-alpha) as well as lymphocyte marker CD4, CD8 and CD19 molecules were increased (P < 0.05) in spleen. Consistently, serum concentrations of IL-2, IL-4, IL-6, TNF-alpha were increased (P < 0.05) in rams following immunization. Similarly, deprivation of testosterone by surgical castration also increased (P < 0.05) GnRH and thus immune cytokine expressions in spleen. Collectively, our data suggested that immunocastration increased GnRH production in spleen by abrogating the inhibitory feedback effects from testosterone, consequently improving the immune markers of spleen and serum immune cytokines in rams. (C) 2016 Elsevier B.V. All rights reserved.

分类号: R96

  • 相关文献

[1]Effect of immunization against GnRH on hypothalamic and testicular function in rams. Han, Xingfa,Gu, Longjun,Cao, Xiaohan,Du, Xiaogang,Zeng, Xianyin,Xia, Chenyang,Feng, Jing,Song, Tianzeng.

[2]Dietary daidzein influences laying performance of ducks (Anas platyrhynchos) and early post-hatch growth of their hatchlings by modulating gene expression. Zhao, RQ,Wang, YJ,Zhou, YC,Ni, YD,Lu, LZ,Grossmann, R,Chen, J.

[3]The vitamin D receptor localization and mRNA expression in ram testis and epididymis. Jin, Hui,Huang, Yang,Qin, Xiaowei,Yao, Xiaolei,Yue, Wenbing,Jin, Guang,Xue, Yanrong.

[4]Expression and localization of guanine nucleotide-binding protein alpha S in the testis and epididymis of rams at different developmental stages. Li, Zhen,Lu, Jieli,Pang, Quanhai,Nan, Ruipeng,Chen, Jia,Zhu, Zhiwei.

[5]Transcriptome analysis of the spleen of the darkbarbel catfish Pelteobagrus vachellii in response to Aeromonas hydrophila infection. Wen, Zhengyong,Yuan, Dengyue,Shao, Ting,Wang, Jun,Li, Huatao,Gong, Quan. 2017

[6]Transcriptional changes of mouse splenocyte organelle components following acute infection with Toxoplasma gondii. He, Jun-Jun,Ma, Jun,Li, Fa-Cai,Song, Hui-Qun,Xu, Min-Jun,Zhu, Xing-Quan,Ma, Jun,Xu, Min-Jun.

[7]Expression analyses and antiviral properties of the Beijing-You and White Leghorn myxovirus resistance gene with different amino acids at position 631. Du, L. X.,Zhao, G. P.,Zhang, C. S..

[8]GlobalgeneexpressionprofileofchickenspleentissueatalaterstageofIBVinfectionrevealspathogenesisandimmunemechanisms. FengCong,ZongxiHan,FengjieWang,YuhaoShao,XiangangKong,ShengwangLiu. 2014

[9]Effects of aluminum trichloride on the trace elements and cytokines in the spleen of rats. Zhu, Yanzhu,Li, Yanfei,Zhu, Yanzhu,Miao, Liguang,Li, Xinwei,Chen, Chongxiao,Wang, Fan,Li, Jing,Hu, Chongwei. 2012

[10]Splenic microRNA Expression Profiles and Integration Analyses Involved in Host Responses to Salmonella enteritidis Infection in Chickens. Li, Peng,Fan, Wenlei,Li, Qinghe,Wang, Jie,Liu, Ranran,Liu, Jie,Zheng, Maiqing,Cui, Huanxian,Zhao, Guiping,Wen, Jie,Li, Peng,Everaert, Nadia,Li, Peng,Wang, Jie,Liu, Jie,Wen, Jie,Zhang, Yonghong. 2017

[11]Effects of Al on the splenic immune function and NE in rats. Hu, Chongwei,Li, Jing,Hu, Chongwei,Zhu, Yanzhu,Bai, Chongsheng,Zhang, Jihong,Xia, Shiliang,Li, Yanfei,Zhu, Yanzhu. 2013

[12]Analysis of miRNA expression profiling in mouse spleen affected by acute Toxoplasma gondii infection. He, Jun-Jun,Ma, Jun,Wang, Jin-Lei,Xu, Min-Jun,Zhu, Xing-Quan,Ma, Jun,Xu, Min-Jun,Zhu, Xing-Quan.

[13]Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels. Li, Guangyu,Qiao, Qin,He, Yan,Zhang, Xuezhen,Yan, Wei,Chen, Jun,Cai, Fei. 2012

[14]Transcriptomic analysis of global changes in cytokine expression in mouse spleens following acute Toxoplasma gondii infection. He, Jun-Jun,Ma, Jun,Song, Hui-Qun,Zhou, Dong-Hui,Wang, Jin-Lei,Huang, Si-Yang,Zhu, Xing-Quan,Ma, Jun,Zhu, Xing-Quan.

[15]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[16]Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis. Chen, Xiao-Qing,Hu, Gui-Xue,Chen, Xiao-Qing,Zhou, Chun-Xue,He, Shuai,Zhu, Xing-Quan,Zhou, Chun-Xue,Elsheikha, Hany M.,He, Shuai. 2017

[17]Analysis of immune-relevant genes expressed in red sea bream (Chrysophrys major) spleen. Chen, SL,Xu, MY,Hu, SN,Li, L.

[18]Novel MicroRNA Involved in Host Response to Avian Pathogenic Escherichia coli Identified by Deep Sequencing and Integration Analysis. Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Lamont, Susan J.,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Jia, Xinzheng,Nie, Qinghua,Zhang, Xiquan,Nolan, Lisa K..

[19]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[20]Boron Affects Immune Function Through Modulation of Splenic T Lymphocyte Subsets, Cytokine Secretion, and Lymphocyte Proliferation and Apoptosis in Rats. Jin, Erhui,Li, Shenghe,Ren, Man,Hu, Qianqian,Gu, Youfang,Li, Shenghe,Li, Kui.

作者其他论文 更多>>