Identification and characterization of two CYP9A genes associated with pyrethroid detoxification in Locusta migratoria

文献类型: 外文期刊

第一作者: Zhu, Wenya

作者: Zhu, Wenya;Yu, Rongrong;Wu, Haihua;Zhang, Xueyao;Liu, Yaoming;Zhang, Jianzhen;Ma, Enbo;Zhu, Wenya;Zhu, Kun Yan;Zhu, Wenya;Zhu, Wenya

作者机构:

关键词: Cytochrome P450 gene;Pyrethroid;Detoxification;RNA interference;Locusta migratoria

期刊名称:PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY ( 影响因子:3.963; 五年影响因子:4.454 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Cytochrome P450s (CYPs) constitute one of the largest gene super families and distribute widely in all living organisms. In this study, the full-length cDNA sequences of two LmCYP9A genes (LmCYP9AQ1 and LmCYP9A3) were cloned from Locusta migratoria. We analyzed the expression patterns of two LmCYP9A genes in various tissues and different developmental stages using real-time quantitative PCR. Then we evaluated the detoxification functions of the two LmCYP9A genes by testing mortalities with four kinds of pyrethroid treatment after RNA interference (RNAi), respectively. Combining with docking structure of two LmCYP9A genes, their detoxification properties were extensively analyzed. The full-length cDNAs of LmCYP9AQ1 and LmCYP9A3 putatively encoded 525 and 524 amino acid residues, respectively. Both LmCYP9A genes were expressed throughout the developmental stages. The expression of LmCYP9AQ1 in the brain was higher than that in other examined tissues, whereas the LmCYP9A3 was mainly expressed in the fat body. The mortalities of nymphs exposed to deltamethrin and permethrin increased from 27.7% to 77.7% and 27.7% to 583%, respectively, after dsLmCYP9A3 injection. While the mortalities of nymphs exposed to fluvalinate increased from 29.8% to 53.0% after LmCYP9AQ1 was silenced using RNA interference. Our results suggested that the two LmCYP9A genes may be involved in different pyrethroid insecticide detoxification in L migratoria. (C) 2016 Elsevier Inc. All rights reserved.

分类号: S4

  • 相关文献

[1]A double-stranded RNA degrading enzyme reduces the efficiency of oral RNA interference in migratory locust. Song, Huifang,Zhang, Jianqin,Li, Daqi,Li, Tao,Liu, Xiaojian,Ma, Enbo,Zhang, Jianzhen,Song, Huifang,Zhang, Jianqin,Li, Daqi,Cooper, Anastasia M. W.,Silver, Kristopher,Liu, Xiaojian,Zhu, Kun Yan.

[2]Synthesis of chrysanthemic amide containing lidinone-ring. Zhang, YQ,Lu, ZK,Li, GL,Guo, SH,Chen, BX. 2001

[3]Individual and Joint Acute Toxicities of Selected Insecticides Against Bombyx mori (Lepidoptera: Bombycidae). Yu, R. X.,Wang, Y. H.,Hu, X. Q.,Wu, S. G.,Cai, L. M.,Zhao, X. P..

[4]Frequencies of the M918I mutation in the sodium channel of the diamondback moth in China, Thailand and Japan and its association with pyrethroid resistance. Wari, David,Shi, Xueyan,Song, Dunlun,Liang, Pei,Gao, Xiwu,Zhang, Youjun,Li, Jianhong,Wu, Gang,Liu, Yong,Li, Ming,Matsumura, Masaya,Minakuchi, Chieka,Tanaka, Toshiharu,Miyata, Tadashi. 2012

[5]Isolation of broad-specificity domain antibody from phage library for development of pyrethroid immunoassay. Zhao, Yanyan,Zhao, Shengming,Tu, Kang,Liang, Ying,Liu, Yuan,Zhang, Xiao,Hu, Xiaodan,Wu, Aihua,Zhang, Cunzheng,Zhong, Jianfeng,Liu, Xianjin,Liang, Ying,Liu, Yuan,Zhang, Xiao,Hu, Xiaodan,Wu, Aihua,Zhang, Cunzheng,Zhong, Jianfeng,Liu, Xianjin,Tu, Sicong.

[6]Isolation, identification and cyfluthrin-degrading potential of a novel Lysinibacillus sphaericus strain, FLQ-11-1. Hu, Gui Ping,Zhao, Yan,Song, Feng Qing,Vasseur, Liette,Douglas, Carl,You, Min Sheng,Hu, Gui Ping,Zhao, Yan,Song, Feng Qing,You, Min Sheng,Liu, Bo,Vasseur, Liette,Douglas, Carl.

[7]Transcriptomic and proteomic analysis of Locusta migratoria eggs at different embryonic stages: Comparison for diapause and non-diapause regimes. Hao Kun,Wang Jie,Tu Xiong-bing,Zhang Ze-hua,Whitman, Douglas W.. 2017

[8]Two distinctive beta subunits are separately involved in two binding sites of imidacloprid with different affinities in Locusta migratoria manilensis. Bao, Haibo,Liu, Yang,Zhang, Yixi,Liu, Zewen,Bao, Haibo. 2017

[9]Mitochondrial genomes reveal the global phylogeography and dispersal routes of the migratory locust. Yang, Pengcheng,Jiang, Feng,Kang, Le,Chapuis, Marie-Pierre,Shali, Yasen,Sword, Gregory A..

[10]Identification of pheromone-like compounds in male reproductive organs of the oriental locust Locusta migratoria. Napolitano, Elio,Serra, Andrea,Iovinella, Immacolata,Pelosi, Paolo,Zhou, Xianhong.

[11]20-Hydroxyecdysone activates PGRP-SA mediated immune response in Locusta migratoria. Han, Pengfei,Han, Jiao,Zhang, Min,Ma, Enbo,Zhang, Jianzhen,Fan, Jigiao,Fan, Renjun,Han, Pengfei,Han, Jiao,Fan, Jigiao,Zhang, Min,Ma, Enbo,Fan, Renjun,Zhang, Jianzhen,Li, Sheng.

[12]Identification of cytochrome P450 monooxygenase genes and their expression profiles in cyhalothrin-treated Colorado potato beetle, Leptinotarsa decemlineata. Wan, Pin-Jun,Shi, Xiao-Qin,Kong, Ye,Zhou, Li-Tao,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun. 2013

[13]Arsenite Resistance, Accumulation, and Volatilization Properties of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1. Feng, Qiufen,Zhang, Yangzhu,Lin, Zhiling,Feng, Qiufen,Su, Shiming,Zeng, Xibai,Li, Lianfang,Bai, Lingyu,Duan, Ran,Lin, Zhiling. 2015

[14]Identification of glutathione S-transferases in Bemisia tabaci (Hemiptera: Aleyrodidae) and evidence that GSTd7 helps explain the difference in insecticide susceptibility between B.tabaci Middle East-Minor Asia 1 and Mediterranean. He, C.,He, C.,Xie, W.,Yang, X.,Wang, S. -L.,Wu, Q. -J.,Zhang, Y. -J.. 2018

[15]Detoxification of diphenyl ether herbicide lactofen by Bacillus sp Za and enantioselective characteristics of an esterase gene lacE. Zhang, Jing,Lu, Luyao,Chen, Feng,Chen, Lingling,Huang, Xing,Zhang, Jing,Yin, Jingang. 2018

[16]Detoxification of Aflatoxin B-1 by Zygosaccharomyces rouxii with Solid State Fermentation in Peanut Meal. Zhou, Guanghui,Chen, Yujie,Kong, Qing,Ma, Yunxiao,Liu, Yang. 2017

[17]Differential physiological effects of neonicotinoid insecticides on honey bees: A comparison between Apis mellifera and Apis cerana. Li, Zhiguo,Li, Meng,He, Jingfang,Zhao, Xiaomeng,Huang, Wei-Fone,Nie, Hongyi,Zhao, Yazhou,Su, Songkun,Chaimanee, Veeranan,Zhao, Yazhou. 2017

[18]Secretory expression and characterization of a novel peroxiredoxin for zearalenone detoxification in Saccharomyces cerevisiae. Wu, Hui,Yu, Yuanshan. 2013

[19]The role of cysteine conjugation in the detoxification of microcystin-LR in liver of bighead carp (Aristichthys nobilis): a field and laboratory study. Zhang, Dawen,Yang, Qiang,Xie, Ping,Deng, Xuwei,Chen, Jun,Dai, Ming,Zhang, Dawen. 2012

[20]Effect of ozone on aflatoxins detoxification and nutritional quality of peanuts. Chen, Ran,Ma, Fei,Li, Pei-Wu,Zhang, Wen,Ding, Xiao-Xia,Zhang, Qi,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Chen, Ran,Li, Pei-Wu,Zhang, Wen,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Ma, Fei,Li, Pei-Wu,Zhang, Qi,Chen, Ran,Ma, Fei,Li, Pei-Wu,Ding, Xiao-Xia,Zhang, Qi,Li, Min,Wang, Yan-Ru,Xu, Bao-Cheng,Li, Pei-Wu,Zhang, Wen,Ding, Xiao-Xia.

作者其他论文 更多>>