Hybrid Sterility in Rice (Oryza sativa L.) Involves the Tetratricopeptide Repeat Domain Containing Protein

文献类型： 外文期刊

第一作者： Yu, Yang

作者： Yu, Yang;Zhao, Zhigang;Shi, Yanrong;Tian, Hua;Liu, Linglong;Bian, Xiaofeng;Xu, Yang;Shen, Yumin;Wang, Chaolong;Yu, Xiaowen;Wang, Chunming;Jiang, Ling;Wan, Jianmin;Zheng, Xiaoming;Gan, Lu;Zhang, Xin;Guo, Xiuping;Wang, Jiulin;Wan, Jianmin;Ikehashi, Hiroshi

作者机构：

关键词： hybrid sterility;female gamete;tetratricopeptide repeat (TPR);transgenic;rice (Oryza sativa L.)

期刊名称：GENETICS （ 影响因子：4.562； 五年影响因子：4.845 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Intersubspecific hybrid sterility is a common form of reproductive isolation in rice (Oryza sativa L.), which significantly hampers the utilization of heterosis between indica and japonica varieties. Here, we elucidated the mechanism of 57, which specially causes Aus-japonica/indica hybrid female sterility, through cytological and genetic analysis, map-based cloning, and transformation experiments. Abnormal positioning of polar nuclei and smaller embryo sac were observed in F-1 compared with male and female parents. Female gametes carrying S7(cp) and S7' were aborted in S7(ai)/S7(cp) and S7(ai)/S7(i), respectively, whereas they were normal in both N22 and Dular possessing a neutral allele, S7(n). S7 was fine mapped to a 139-kb region in the centromere region on chromosome 7, where the recombination was remarkably suppressed due to aggregation of retrotransposons. Among 16 putative open reading frames (ORFs) localized in the mapping region, ORF3 encoding a tetratricopeptide repeat domain containing protein was highly expressed in the pistil. Transformation experiments demonstrated that ORF3 is the candidate gene: downregulated expression of ORF3 restored spikelet fertility and eliminated absolutely preferential transmission of S7(ai) in heterozygote S7(ai)/S7(cp); sterility occurred in the transformants Cpslo17-S7(ai). Our results may provide implications for overcoming hybrid embryo sac sterility in intersubspecific hybrid rice and utilization of hybrid heterosis for cultivated rice improvement.

分类号： R394

  • 相关文献

[1]Identification of a new hybrid sterility gene in rice (Oryza sativa L.). Zhao, Zhigang,Wang, Chunming,Jiang, Ling,Zhu, Susong,Ikehashi, Hiroshi,Wan, Jianmin. 2006

[2]Molecular mapping of a pollen killer gene S29(t) in Oryza Glaberrima and co-linear analysis with S22 in O-Glumaepatula. Hu, Fengyi,Xu, Peng,Deng, Xianneng,Zhou, Jiawu,Li, Jing,Tao, Dayun. 2006

[3]The role of S1-g allele from Oryza glaberrima in improving interspecific hybrid sterility between O. sativa and O. glaberrima. Deng, Xianneng,Zhou, Jiawu,Xu, Peng,Li, Jing,Hu, Fengyi,Tao, Dayun,Deng, Xianneng. 2010

[4]Developing rice lines possessing neutral alleles at sterility loci to improve the width of compatibility. Lu, CG,Zou, JS,Ikehashi, H. 2004

[5]Fine mapping of pss1, a pollen semi-sterile gene in rice (Oryza sativa L.). Li, Wanchang,Jiang, Ling,Zhou, Shirong,Wang, Chunming,Liu, Linglong,Chen, Liangming,Ikehashi, Hiroshi,Wan, Jianmin.

[6]Fine mapping of S32(t), a new gene causing hybrid embryo sac sterility in a Chinese landrace rice (Oryza sativa L.). Chen, Liangming,Jiang, Ling,Zhu, Susong,Zhao, Zhigang,Liu, Shijia,Su, Ning,Zhai, Huqu,Ikehashi, Hiroshi,Wan, Jianmin.

[7]Fine mapping of a gene causing hybrid pollen sterility between Yunnan weedy rice and cultivated rice (Oryza sativa L.) and phylogenetic analysis of Yunnan weedy rice. Wang, Yong,Zhong, Zheng Zheng,Zhao, Zhi Gang,Jiang, Ling,Bian, Xiao Feng,Zhang, Wen Wei,Liu, Ling Long,Wan, Jian Min,Wan, Jian Min,Ikehashi, H..

[8]Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.). Zhao, Z. G.,Zhang, Y. H.,Bian, X. F.,Wang, Y.,Jiang, L.,Liu, X.,Chen, L. M.,Liu, S. J.,Zhang, W. W.,Wan, J. M.,Zhu, S. S.,Wan, J. M.,Ikehashi, H..

[9]Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O-glaberrima Steud. Shen, Yumin,Zhao, Zhigang,Ma, Hongyang,Bian, Xiaofeng,Yu, Yang,Yu, Xiaowen,Chen, Haiyuan,Liu, Linglong,Zhang, Wenwei,Jiang, Ling,Wan, Jianmin,Wan, Jianmin,Zhou, Jiawu,Tao, Dayun.

[10]The origin of weedy rice Ludao in China deduced by genome wide analysis of its hybrid sterility genes. Zhu, SS,Jiang, L,Wang, CM,Zhai, HQ,Li, DT,Wan, JM. 2005

[11]A genome-wide analysis of wide compatibility in rice and the precise location of the S-5 locus in the molecular map. Liu, KD,Wang, J,Li, HB,Xu, CG,Liu, AM,Li, XH,Zhang, QF. 1997

[12]Mapping QTLs for hybrid sterility in three AA genome wild species of Oryza. Yang, Ying,Zhou, Jiawu,Li, Jing,Xu, Peng,Zhang, Yu,Tao, Dayun.

[13]Fine mapping of a gene responsible for pollen semi-sterility in hybrids between Oryza sativa L. and O. glaberrima Steud. Wang, Jiankang,Wan, Jianmin,Zhang, Yunhui,Zhao, Zhigang,Zhou, Jiawu,Jiang, Ling,Bian, Xiaofeng,Wang, Yong,Wang, Chaolong,Zhong, Zhengzheng,Wan, Jianmin,Zhou, Jiawu,Tao, Dayun.

[14]A new gene controlling hybrid sterility between Oryza sativa and Oryza longistaminata. Li, Jing,Xu, Peng,Zhou, Jiawu,Hu, Fengyi,Deng, Xianneng,Deng, Wei,Tao, Dayun,Zhao, Jiying.

[15]Preliminary Study on the Physiological Characteristics of Transgenic Wheat with Maize C-4-pepc Gene in Field Conditions. Han, L. L.,Han, L. L.,Xu, W. G.,Hu, L.,Li, Y.,Qi, X. L.,Zhang, J. H.,Zhang, H. F.,Wang, Y. X.. 2014

[16]The sense and antisense expression of gibberellin 20-oxidase gene (rga5) in rice and its effects on GA(1) level and agronomic traits. Yan, YX,An, CG,Li, L,Gu, JY,Wang, LJ,Mi, SL,Chen, ZL. 2003

[17]Pest control and resistance management through release of insects carrying a male-selecting transgene. Harvey-Samuel, Tim,Alphey, Nina,Alphey, Luke,Harvey-Samuel, Tim,Morrison, Neil I.,Walker, Adam S.,Marubbi, Thea,Gorman, Kevin,Warner, Simon,Alphey, Luke,Yao, Ju,Collins, Hilda L.,Shelton, Anthony M.,Yao, Ju,Davies, T. G. Emyr,Alphey, Nina,Alphey, Luke. 2015

[18]A Non-destructive Terahertz Spectroscopy-Based Method for Transgenic Rice Seed Discrimination via Sparse Representation. Hu, Xiaohua,Zheng, Lei,Hu, Xiaohua,Lang, Wenhui,Liu, Wei,Xu, Xue,Yang, Jianbo. 2017

[19]The study on anthocyanin regulatory genes as a visual marker in transformation of common wheat (Triticum aestivum). He Yanli,Zhang Xiaodong,He Yanli,Yang Fengping,Zhang Xiaodong. 2014

[20]Inhibition of Newcastle Disease Virus Replication by Lentivirus-Mediated RNA Interference. Wang, Ze,Ding, Zhuang,Dang, Yuan,Guo, Yupei,Yang, Jianxin,Cong, Yanlong,Ding, Chan,Yu, Shengqing,Meng, Qingfeng,Liu, Jinhua.

作者其他论文 更多>>

微信小程序