(-)-Epigallocatechin gallate (EGCG)-nanoethosomes as a transdermal delivery system for docetaxel to treat implanted human melanoma cell tumors in mice

文献类型: 外文期刊

第一作者: Liao, Bingwu

作者: Liao, Bingwu;Ying, Hao;Fan, Zhaoyang;Zhang, Weihua;Ravichandran, Nagaiya;Du, Qizhen;Yu, Chenhuan;Ying, Huazhong;Shi, John;Xu, Yongquan;Yin, Junfeng;Jiang, Yongwen

作者机构:

关键词: (-)-Epigallocatechin-3-O-gallate;Nanoethosomes;Transdermal delivery;Docetaxel;Skin cancer;Pharmacokinetics

期刊名称:INTERNATIONAL JOURNAL OF PHARMACEUTICS ( 影响因子:5.875; 五年影响因子:5.423 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: (-)-Epigallocatechin-3-O-gallate (EGCG), a versatile natural product in fresh tea leaves and green tea, has been investigated as a preventative treatment for cancers and cardiovascular disease. The objective of this study was to develop EGCG-nanoethosomes for transdermal delivery and to evaluate them for treating subcutaneously implanted human melanoma cell tumors. EGCG-nanoethosomes, composed of 0.2% EGCG, 2% soybean phosphatidylcholine, 30% ethanol, 1% Tween-80 and 0.1% sugar esters, were prepared and characterized using laser transmission electron microscopy. These nanoethosomes were smoother and more compact than basic-nanoethosomes with the same components except for EGCG. The effectiveness of transdermal delivery by EGCG-nanoethosomes was demonstrated in an in vitro permeability assay system using mouse skin. The inhibitory effect of docetaxel (DT) loaded in EGCG-nanoethosomes (DT-EGCG-nanoethosomes) was analyzed by monitoring growth of a subcutaneously implanted tumor from A-375 human melanoma cells in mice. Mice treated with DT-EGCG-nanoethosomes exhibited a significant therapeutic effect, with tumors shrinking, on average, by 31.5% of initial volumes after 14 d treatment. This indicated a potential for treating skin cancer. In a pharmacokinetic study, transdermal delivery by DT-EGCG-nanoethosomes enabled sufficient DT exposure to the tumor. Together, these findings indicated that EGCG-nanoethosomes have great potential as drug carriers for transdermal delivery. (C) 2016 Elsevier B.V. All rights reserved.

分类号: R9

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