Effects of different promoters on the protective efficacy of recombinant Marek's disease virus type 1 expressing the VP2 gene of infectious bursal disease virus

文献类型： 外文期刊

第一作者： Li, Kai

作者： Li, Kai;Liu, Yongzhen;Liu, Changjun;Gao, Li;Zhang, Yanping;Gao, Yulong;Cui, Hongyu;Qi, Xiaole;Zhong, Li;Wang, Xiaomei

作者机构：

关键词： Recombinant MDV1;Infectious bursal disease virus;Promoters;VP2 protein;Protective efficacy

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

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年卷期：

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收录情况： SCI

摘要： The vaccine efficacy of recombinant viruses can be influenced by many factors. Accordingly, the activity of promoters has been one of the major factors affecting the antigen expression and protection rate. In the present study, two recombinant Marek's disease virus type 1 (MDV1) vaccines containing the VP2 gene of infectious bursal disease virus (IBDV) under control of different promoters were generated from overlapping fosmid DNAs. The rMDV-Pec-VP2 virus containing the VP2 gene under control of the Pec promoter (CMV enhancer and chicken beta-actin chimera promoter) demonstrated higher VP2 expression and stronger antibody response against IBDV in chickens than the rMDV-CMV-VP2 virus using the CMV promoter. After IBDV lethal challenge in specific-pathogen-free chickens, rMDV-Pec-VP2 provided complete protection against developing mortality, clinical signs, and the formation of bursal lesions, which was better than that provided by rMDV-CMV-VP2. Our findings indicate that the protective efficacy of the recombinant MDV1 vaccine against IBDV highly correlates with VP2 expression. This recombinant MDV1 vaccine expressing VP2 could have significant potential as a bivalent vaccine against both virulent IBDV and MDV infections in chickens. (C) 2016 Elsevier Ltd. All rights reserved.

分类号： R392

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