Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation
文献类型: 外文期刊
第一作者: Wang, Xiaoxu
作者: Wang, Xiaoxu;Li, Shiyu;Lu, Xiaoran;Li, Zheng;Bu, Zhaoyang;Lang, Xulong;Wang, Xinlong;Wang, Xiaoxu;Hu, Pan;Chen, Haiyan
作者机构:
关键词: quorum sensing;gene inactivation;biofilm formation;auto-inducer 2
期刊名称:MOLECULAR MEDICINE REPORTS ( 影响因子:2.952; 五年影响因子:2.754 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Rapid and efficient inactivation of a target gene in Escherichia coli chromosomes is required to investigate metabolic engineering. In the present study, a multiple gene inactivation approach was demonstrated in four strains of enterotoxigenic E. coli (ETEC), which are the predominant pathogenic bacteria causing piglet diarrhea, mediated by lambda Red and Xer recombination. The chromosomal genes, luxS and pfs were inactivated using the multiple gene inactivation approach in the wild-type strains of E. colt, K88,K 99, 987 P and F41. This indicated that dif sites may be reused to inactivate multiple chromosomal genes when no antibiotic-resistant selectable markers remain. Following inactivation of luxS and pfs, the ability of ETEC to produce the quorum sensing signal, and induce auto-inducer 2 activity and biofilm formation were significantly reduced. Furthermore, the multiple gene inactivation approach also exhibits a high recombination efficiency and follows a simple process.
分类号: R3
- 相关文献
作者其他论文 更多>>
-
Map-based cloning revealed BhAPRR2 gene regulating the black peel formation of mature fruit in wax gourd (Benincasa hispida)
作者:Zhai, Xuling;Yan, Jinqiang;Liu, Wenrui;Cao, Zhenqiang;Mo, Renlian;Wang, Baochen;Cheng, Xiaoxin;Xie, Dasen;Jiang, Biao;Li, Zheng;Deng, Ying
关键词:
-
Converse effects of warming and reclamation on methane uptake in alpine meadows
作者:Huang, Jing;Hu, Guozheng;Ganjurjav, Hasbagan;Gao, Qingzhu;Li, Zheng;Ji, Guoxu;Huang, Jing;Li, Zheng;Ji, Guoxu;Hu, Guozheng;Gao, Qingzhu;Ganjurjav, Hasbagan;Sha, Yubao;Liu, Huihui;Han, Ling;He, Shicheng;Yan, Jun;Wang, Youxia;Xie, Wendong;Gornish, Elise S.
关键词:Warming; Reclamation; Alpine meadows; Cultivated grassland; Qinghai-Tibet Plateau; Methane
-
The utilization of an ultrasonic mung bean protein-starch conjugate as a fat substitute in whipping cream
作者:Feng, Xuewei;Li, Shiyu;Jiang, Chunyang;Su, Hang;Yao, Yang;Feng, Xuewei;Zhu, Yingying;Zhang, Yongjiu;Hao, Xiyu;Hao, Xiyu
关键词:ultrasound; mung bean protein-starch conjugate; whipping cream; physicochemical properties; in vitro digestibility
-
CsIREH1 phosphorylation regulates DELLA protein affecting plant height in cucumber (Cucumis sativus)
作者:Zhao, Hongjiao;Sun, Piaoyun;Tong, Can;Li, Xiangbao;Yang, Tongwen;Jiang, Yanxin;Zhao, Bosi;Dong, Junyang;Shen, Junjun;Li, Zheng;Sun, Piaoyun;Jiang, Biao
关键词:AGC protein kinase; CsIREH1; cucumber; DELLA protein; plant height
-
Molecular mechanisms of cis-oxygen bridge neonicotinoids to Apis mellifera Linnaeus chemosensory protein: Surface plasmon resonance, multiple spectroscopy techniques, and molecular modeling
作者:Li, Xiangshuai;Li, Shiyu;Liu, Yang;Cui, Li;Yang, Daibin;Chen, Shuning;Yuan, Huizhu;Yan, Xiaojing;Shao, Xusheng
关键词:Chemosensory protein; Neonicotinoid; Surface plasmon resonance; Multispectroscopy; Molecular modeling
-
Physicochemical properties and fungal communities of forest soil combine to influence ginseng rusty root grade
作者:Qiao, Siwei;Xu, Hang;Li, Zheng;Xu, Shiquan;Zhang, Hao;Qiao, Siwei;Xu, Hang;Li, Zheng;Xu, Shiquan;Zhang, Hao;Chen, Xiaolin;Li, Shuxin
关键词:Forest soil properties; Fungal community; Ginseng rusty root disease; Ilyonectria spp.; Mortierellomycota
-
Metagenomic Investigation of Pathogenic RNA Viruses Causing Diarrhea in Sika Deer Fawns
作者:Wang, Weiyang;Wang, Qilin;Cao, Runlai;Li, Yacong;Liu, Ziyu;Xue, Zhuqing;Wang, Xiaoxu;Liu, Zhijie
关键词:sika deer; viral metagenomics; whole-genome sequencing; rotavirus