Rapid method of luxS and pfs gene inactivation in enterotoxigenic Escherichia coli and the effect on biofilm formation

文献类型: 外文期刊

第一作者: Wang, Xiaoxu

作者: Wang, Xiaoxu;Li, Shiyu;Lu, Xiaoran;Li, Zheng;Bu, Zhaoyang;Lang, Xulong;Wang, Xinlong;Wang, Xiaoxu;Hu, Pan;Chen, Haiyan

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关键词: quorum sensing;gene inactivation;biofilm formation;auto-inducer 2

期刊名称:MOLECULAR MEDICINE REPORTS ( 影响因子:2.952; 五年影响因子:2.754 )

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收录情况: SCI

摘要: Rapid and efficient inactivation of a target gene in Escherichia coli chromosomes is required to investigate metabolic engineering. In the present study, a multiple gene inactivation approach was demonstrated in four strains of enterotoxigenic E. coli (ETEC), which are the predominant pathogenic bacteria causing piglet diarrhea, mediated by lambda Red and Xer recombination. The chromosomal genes, luxS and pfs were inactivated using the multiple gene inactivation approach in the wild-type strains of E. colt, K88,K 99, 987 P and F41. This indicated that dif sites may be reused to inactivate multiple chromosomal genes when no antibiotic-resistant selectable markers remain. Following inactivation of luxS and pfs, the ability of ETEC to produce the quorum sensing signal, and induce auto-inducer 2 activity and biofilm formation were significantly reduced. Furthermore, the multiple gene inactivation approach also exhibits a high recombination efficiency and follows a simple process.

分类号: R3

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