Characterization of a New Wheat-Aegilops biuncialis 1M(b)(1B) Substitution Line with Good Quality-associated HMW Glutenin Subunit

文献类型： 外文期刊

第一作者： Cheng, Y.

作者： Cheng, Y.;Zang, L. L.;Zheng, X. L.;Deng, K. J.;Zhang, Y.;Yang, E. N.;Liu, C.;Zhu, Y. Q.

作者机构：

关键词： wheat;Aegilops biuncialis;bread-making quality;HMW-GS;multi-colour FISH

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

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收录情况： SCI

摘要： In this study, a new substitution line, 12-5-1, with 42 chromosomes that was derived from BC3F2 descendants of the hybridization between Triticum aestivum cv. CN19 and Aegilops biuncialis was created and reported. The 12-5-1 was immune to both powdery mildew and stripe rust and has stable fertility. Multi-color fluorescence in situ hybridization indicated that 12-5-1 was a substitution line 1M(b)(1B). The seed storage protein electrophoresis showed that 12-5-1 presented high molecular weight glutenin subunits (2 + 12) of CN19 and a new subunit designated as M which apparently originated from parent Ae. biuncialis, and absent 7 + 8 subunits. Additionally, the flour quality parameters showed that the protein content, Zeleny sedimentation value, wet gluten content, and grain hardness and mixing time of 12-5-1 were signifiantly higher than those of its parent CN19. Moreover, 5 pairs of the chromosome 1M(b)-specifi polymerase chain reaction-based landmark unique gene markers, TNAC1021, TNAC1026, TNAC1041, TNAC1-02 and TNAC1-04, were also obtained. The new substitution line 1M(b)(1B) 12-5-1 could be a valuable source for wheat improvement, especially for wheat end product quality and resistance to disease.

分类号： S5

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