Morphological and Physiological Responses of Sugarcane to Leifsonia xyli subsp xyli Infection

文献类型： 外文期刊

第一作者： Chen, Minghui

作者： Chen, Minghui;Liang, Yongjian;Xing, Yongxiu;Yang, Litao;Chen, Minghui;Comstock, Jack C.;Li, Yangrui;Yang, Litao;Li, Yangrui;Yang, Litao

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期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

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收录情况： SCI

摘要： Ratoon stunt, caused by the bacterium Leifsonia xyli subsp. xyli, is one of the major sugarcane diseases worldwide. The objectives of this study were to determine the variation in morphology and DNA sequence of L. xyli subsp. xyli strains isolated in China, to compare the changes that occurred in vascular ultrastructure and levels of endogenous hormone abscisic acid (ABA), auxins (indoleacetic acid [IAA]), and gibberellic acids (GA(3)) in sugarcane stalks Experiments were also conducted with two sugarcane varieties, 'ROC22' and 'Badila', in the greenhouse to understand the cytological and physiological mechanisms of L. xyli subsp. xyli induced growth stunting. There were three treatments in the experiments: (i) healthy plants (L. xyli subsp. xyli free plants), (ii) infected plants (L. xyli subsp. xyli infected seedcanes treated with hot water, and (iii) infected plants (healthy seedcanes dipped in L. xyli subsp. xyli cell culture). The results showed that sequence coverage of a locally isolated strain, LxxGXBZ01, was 99.99%, and the average nucleotide identity between LxxGXBZ01 and the other well-characterized Brazilian isolate LxxCTCB07 was 93.61%. LxxGXBZ01 occurred in different sizes and shapes in xylem vessels of infected plants. In comparison with healthy stalks, the secondary walls of the vessel element in L. xyli subsp. xyli infected stalks were degraded with uneven wall thickness, deformities, sticky substances, and electron-dense substances accumulated inside the cells. Compared with the healthy and hot-water treatments, the contents of IAA and GA(3) were significantly lower, while that of ABA was significantly higher in the L. xyli subsp. xyli infected stalks. The information obtained in this study will expand our understanding of ratoon stunt etiology and cytological and physiological bases of the disease manifestation.

分类号： S4

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