Bone marrow-derived mesenchymal stem cells in three-dimensional culture promote neuronal regeneration by neurotrophic protection and immunomodulation

文献类型： 外文期刊

第一作者： Han, Sufang

作者： Han, Sufang;Li, Xing;Xiao, Zhifeng;Han, Jin;Zhao, Yannan;Yin, Yanyun;Chen, Bing;Dai, Jianwu;Wang, Bin;Li, Xing;Fang, Yongxiang

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关键词： three-dimensional culture;bone marrow derived mesenchymal stem cells;neurotrophic factors;inflammatory cytokines;spinal cord injury

期刊名称：JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A （ 影响因子：4.396； 五年影响因子：4.205 ）

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收录情况： SCI

摘要： Accumulating evidence has revealed three-dimensional (3D) culture could better mimic the stem cell niche in vivo in comparison with conventional two-dimensional (2D) culture. In this study, we found that bone marrow derived mesenchymal stem cells (BMSCs) cultured in 3D collagen scaffold (3D BMSCs) exhibited distinctive features including significantly enhancing neurotrophic factor secretions and reducing macrophage activations challenged by lipopolysaccharide (LPS) in vitro. To further evaluate 3D BMSCs' potential benefits to the regeneration of spinal cord injury (SCI), the 3D and 2D BMSCs were respectively implanted in rat hemisected SCI. Compared with 2D cohort, 3D BMSCs transplantation significantly reduced the expressions of inflammatory cytokines such as TNF-, IL-1, and IL-6 at 5 days after transplantation, markedly enhanced axonal regeneration, and promoted motor functional recovery during 8 weeks of observation. When Nocodazole was used to depolymerize the cytoskeleton of 3D BMSCs, the changed expressions of neurotrophic factors and inflammatory cytokines were blunted, at least partially. Thus synergistic effects of neuronal protection and immunomodulation of 3D BMSCs may lead to a better functional recovery of SCI and the underlying mechanism may involve the alteration of their cellular morphology because of 3D culture. This study contributes to a better understanding of the cellular characteristics of 3D BMSCs and provides a novel strategy to promote the repair of the injured spinal cord. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1759-1769, 2016.

分类号： R31

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