Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos

文献类型： 外文期刊

第一作者： Zhang, Xuemei

作者： Zhang, Xuemei;Li, Wenrong;Zhang, Fuchun;Liu, Mingjun;Zhang, Xuemei;Wang, Liqin;Wu, Yangsheng;Li, Wenrong;An, Jing;Liu, Mingjun;Zhang, Xuemei;Wang, Liqin;Wu, Yangsheng;Li, Wenrong;An, Jing;Liu, Mingjun

作者机构：

关键词： Myostatin;Zinc-finger Nucleases;Knockout;Sheep

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

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年卷期：

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收录情况： SCI

摘要： Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.

分类号： Q95

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