Interactions of two odorant-binding proteins influence insect chemoreception

文献类型： 外文期刊

第一作者： Sun, X.

作者： Sun, X.;Zeng, F. -F.;Yan, M. -J.;Wang, M. -Q.;Sun, X.;Zhang, A.;Lu, Z. -X.

作者机构：

关键词： odorant-binding protein;Cnaphalocrocis medinalis;molecular docking;fluorescence competitive binding assays;RNA interference;interactions;electroantennogram responses

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： It is well known that the odorant-binding proteins (OBPs) play crucial roles in insect olfactory detection. To explore if interactions of OBPs affect olfactory coding in the rice leaffolder Cnaphalocrocis medinalis ligand-binding experiments, molecular docking, RNA interference and electrophysiological recording were performed. The binding activity of two C. medinalis OBPs (CmedOBPs) to rice plant volatiles showed wide flexibility depending on the structure of ligands and interactions of CmedOBPs involved. The binding sites of CmedOBP2 and CmedOBP3 to rice plant volatiles were well predicted by three-dimensional structure modelling and molecular docking experiments. In addition, the interactions of these two CmedOBPs in the perception of rice volatiles were demonstrated by RNA interference experiments. When a single double-stranded RNA (dsRNA)-Cme-dOBP2 was injected, the expression of CmedOBP2 was significantly reduced and the expression of CmedOBP3 was significantly increased, and vice versa. When both dsRNA-CmedOBP2 and 3 were injected together, greater reduction of electroantennogram responses to rice plant volatiles was induced than that seen with individual injection of either dsRNA-CmedOBP2 or dsRNA-CmedOBP3. These results clearly indicate that the interactions of CmedOBP2 and CmedOBP3 have significant effects on C. medinalis during the detection of host plant volatiles.

分类号： R47

  • 相关文献

[1]Specific involvement of two amino acid residues in cis-nerolidol binding to odorant-binding protein 5 AlinOBP5 in the alfalfa plant bug, Adelphocoris lineolatus (Goeze). Gu, S. -H.,Guo, Y. -Y.,Zhang, Y. -J.,Han, L.,Zhou, J. -J.. 2013

[2]Functional analysis of female-biased odorant binding protein 6 for volatile and nonvolatile host compounds in Adelphocoris lineolatus (Goeze). Sun, L.,Wang, Q.,Wang, Q.,Khashaveh, A.,Zhang, Y. -J.,Guo, Y. -Y.,Wang, Q.,Yang, S.,Zhang, Z..

[3]Functional characterization of a pheromone-binding protein from rice leaffolder Cnaphalocrocis medinalis in detecting pheromones and host plant volatiles. Sun, X.,Zhao, Z. -F.,Zeng, F. -F.,Wang, M. -Q.,Sun, X.,Zhang, A.,Lu, Z. -X..

[4]Ligand-binding properties of three odorant-binding proteins of the diamondback moth Plutella xylostella. Yuan Hai-bin,Zhu Jiao,Pelosi, Paolo,Liu Yang,Lin Ke-jian,Wang Gui-rong. 2016

[5]Sex pheromone recognition and immunolocalization of three pheromone binding proteins in the black cutworm moth Agrotis ipsilon. Gu, Shao-Hua,Wang, Gui-Rong,Zhang, Yong-Jun,Guo, Yu-Yuan,Zhou, Jing-Jiang.

[6]The antenna-specific odorant-binding protein AlinOBP13 of the alfalfa plant bug Adelphocoris lineolatus is expressed specifically in basiconic sensilla and has high binding affinity to terpenoids. Liu, Z-W.,Sun, L.,Xiao, H-J.,Gu, S-H.,Guo, Y-Y.,Zhang, Y-J.,Xiao, H-J.,Zhou, J-J.. 2014

[7]Cloning, expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug, Adelphocoris lineolatus (Goeze). Gu ShaoHua,Sun Yang,Ren LiYan,Zhang XueYing,Zhang YongJun,Wu KongMing,Guo YuYuan,Sun Yang,Ren LiYan. 2010

[8]Transcriptional and Antagonistic Responses of Biocontrol Strain Lysobacter enzymogenes OH11 to the Plant Pathogenic Oomycete Pythium aphanidermatum. Zhao, Yangyang,Liu, Fengquan,Qian, Guoliang,Chen, Yuan,Du, Liangcheng. 2017

[9]Effect of rutin and quercetin on the physicochemical properties of Tartary buckwheat starch. He, Caian,Zhang, Zhen,Liu, Hang,Wang, Min,Gao, Jinfeng,Li, Yunlong. 2018

[10]Effects of sodium gluconate and phytase on performance and bone characteristics in broiler chickers. Guo, Yanli,Shi, Yanghong,Li, Fadi,Zhen, Chen,Hao, Zhengli,Chen, Jilan.

[11]Interactions between foliage- and soil-dwelling predatory mites and consequences for biological control of Frankliniella occidentalis. Gao, Yulin,Xu, Xuenong,Lei, Zhongren,Zhang, Zhike.

[12]Rice ragged stunt oryzavirus: role of the viral spike protein in transmission by the insect vector. Zhou, GY,Lu, XB,Lu, HJ,Lei, JL,Chen, SX,Gong, ZX.

[13]Quantitative investigation of the direct interaction between Hemagglutinin and fusion proteins of Peste des petits ruminant virus using surface Plasmon resonance. Meng, Xuelian,Deng, Ruixue,Zhu, Xueliang,Zhang, Zhidong. 2018

[14]Three odorant binding proteins may regulate the behavioural response of Chrysopa pallens to plant volatiles and the aphid alarm pheromone (E)-beta-farnesene. Z.-Q. Li,Chen, Z. -M.,S. Zhang,X.-M. Cai,J.-Y. Luo,S.-L. Dong,J.-J. Cui,Z.-M. Chen.

[15]Odorant-binding proteins display high affinities for behavioral attractants and repellents in the natural predator Chrysopa pallens. Zhao-Qun Li,Shuai Zhang,Jun-Yu Luo,Si-BaoWang,Shuang-Lin Dong,Jin-Jie Cui.

[16]Conserved chemosensory proteins in the proboscis and eyes of Lepidoptera. Liu, Yang,Pelosi, Paolo,Wang, Guirong,Iovinella, Immacolata,Liu, Yu-Ling,Huang, Ling-Qiao,Wang, Chen-Zhu,Iovinella, Immacolata,Dani, Francesca Romana. 2016

[17]Identification of odorant-binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana. Wang, J.,He, M.,Li, Z. -Q.,Zhang, Y. -F.,Chen, L.,Xu, L.,Zhang, Y. -N.. 2017

[18]Odorant-binding and chemosensory proteins identified in the antennal transcriptome of Adelphocoris suturalis Jakovlev. Gu, Shao-Hua,Zhu, Xiao-Qiang,Wei, Yu,Liu, Hang-Wei,Khalid, Hussain Dhiloo,Guo, Yu-Yuan,Zhang, Yong-Jun,Khalid, Hussain Dhiloo. 2017

[19]CONSTRUCTION AND ANALYSIS OF ANTENNAL cDNA LIBRARY FROM RICE STRIPED STEM BORER, Chilo suppressalis (WALKER) (LEPIDOPTERA: PYRALIDAE), AND EXPRESSION PROFILES OF PUTATIVE ODORANT-BINDING PROTEIN AND CHEMOSENSORY PROTEIN GENES. Gong, Zhong-Jun,Liu, Su,Jiang, Yan-Dong,Zhou, Wen-Wu,Liang, Qing-Mei,Cheng, Jiaan,Zhang, Chuan-Xi,Zhu, Zeng-Rong,Gurr, Geoff M.,Gong, Zhong-Jun,Liu, Su,Jiang, Yan-Dong,Zhou, Wen-Wu,Liang, Qing-Mei,Cheng, Jiaan,Zhang, Chuan-Xi,Zhu, Zeng-Rong,Gurr, Geoff M.,Gong, Zhong-Jun,Liu, Su,Jiang, Yan-Dong,Zhou, Wen-Wu,Liang, Qing-Mei,Cheng, Jiaan,Zhang, Chuan-Xi,Zhu, Zeng-Rong,Gurr, Geoff M.,Gong, Zhong-Jun,Gurr, Geoff M..

[20]Molecular cloning, expression profile, odorant affinity, and stability of two odorant-binding proteins in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae). Zhang, Tiantao,Wang, Zhenying,He, Kanglai,Bai, Shuxiong,Ahmed, Tofael.

作者其他论文 更多>>

微信小程序