High-level expression and biochemical characterization of a novel cold-active lipase from Rhizomucor endophyticus
文献类型: 外文期刊
第一作者: Duan, Xiaojie
作者: Duan, Xiaojie;Liu, Yu;Jiang, Zhengqiang;Yang, Shaoqing;Zheng, Mingming
作者机构:
关键词: Cold active lipase;Gene cloning;Lipase;Organic solvent tolerance;Pichia pastoris;Rhizomucor endophyticus
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
ISSN:
年卷期:
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收录情况: SCI
摘要: A novel cold-active lipase gene (ReLipB) from Rhizomucor endophyticus was cloned. ReLipB was expressed at a high level in Pichia pastoris using high cell-density fermentation in a 5-l fermentor with the highest lipase activity of 1395 U/ml. The recombinant lipase (RelipB) was purified and biochemically characterized. ReLipB was most active at pH 7.5 and 25 A degrees C. It was stable from pH 4.5-9.0. It exhibited broad substrate specificity towards p-nitrophenyl (pNP) esters (C-2-C-16) and triacylglycerols (C-2-C-12), showing the highest specific activities towards pNP laurate (231 U/mg) and tricaprylin (1840 U/mg), respectively. In addition, the enzyme displayed excellent stability with high concentrations of organic solvents including cyclohexane, n-hexane, n-heptane, isooctane and petroleum ester and surfactants.
分类号: Q7
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