CsWRKY46, a WRKY transcription factor from cucumber, confers cold resistance in transgenic-plant by regulating a set of cold-stress responsive genes in an ABA-dependent manner

文献类型： 外文期刊

第一作者： Zhang, Ying

作者： Zhang, Ying;Yu, Hongjun;Yang, Xueyong;Li, Qiang;Ling, Jian;Wang, Hong;Gu, Xingfang;Huang, Sanwen;Jiang, Weijie;Zhang, Ying;Jiang, Weijie

作者机构：

关键词： Abiotic stress;Abscisic acid;Cucumber;Transcription factor;Transgenic plants

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Plant WRKY transcription factors are trans-regulatory proteins that are involved in plant immune responses, development and senescence; however, their roles in abiotic stress are still not well understood, especially in the horticultural crop cucumber. In this study, a novel cucumber WRKY gene, CsWRKY46 was cloned and identified, which was up-regulated in response to cold stress and exogenous abscisic acid (ABA) treatment. CsWRKY46 is belonging to group II of the WRKY family, CsWRKY46 was found exclusively in the nucleus, as indicated by a transient expression assay. Yeast one-hybrid assay shown that CsWRKY46 interact with the W-box in the promoter of ABI5. Transgenic Arabidopsis lines over expressing CsWRKY46, WRK46-OE1 and WRK46-OE5 had higher seedling survival rates upon freezing treatment compared with that of the wild-type. The above over-expression lines also showed much a higher proline accumulation, less electrolyte leakage and lower malondialdehyde (MDA) levels. Furthermore, the CsWRKY46 overexpression lines were hypersensitive to ABA during seed germination, but the seedlings were not. Quantitative RT-PCR analyses revealed that the expression levels of the ABA responsive transcription factor ABI5 were higher in the WRKY46-OE lines than in wild-type and that the overexpression of CsWRKY46 increased the expression of stress-inducible genes, including RD29A and COR47. Taken together, our results demonstrated that CsWRKY46 from cucumber conferred cold tolerance to transgenic plants and positively regulated the cold signaling pathway in an ABA-dependent manner. (C) 2016 Elsevier Masson SAS. All rights reserved.

分类号： Q945`Q946

  • 相关文献

[1]A Novel miRNA Sponge Form Efficiently Inhibits the Activity ofmiR393 and Enhances the Salt Tolerance and ABA Insensitivity in Arabidopsis thaliana. Long, Ruicai,Li, Xiao,Gao, Yanli,Zhang, Tiejun,Kang, Junmei,Cong, Lili,Yang, Qingchuan,Li, Mingna,Sun, Yan,Wang, Tenghua.

[2]Transcription factor OsAP21 gene increases salt/drought tolerance in transgenic Arabidopsis thaliana. Jin, Xiaofeng,Xue, Yong,Xu, RanRan,Bian, Lin,Zhu, Bo,Han, Hongjuan,Peng, Rihe,Yao, Quanhong,Wang, Ren.

[3]Expression of the ethylene response factor gene TSRF1 enhances abscisic acid responses during seedling development in tobacco. Zhang, Hongbo,Zhang, Zhijin,Huang, Rongfeng,Zhang, Hongbo,Chen, Jia,Wang, Xue-Chen,Yang, Yuhong,Zhang, Hongbo,Zhang, Zhijin,Huang, Rongfeng.

[4]Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement. Sun, Yiding,Yu, Diqiu,Sun, Yiding,Sun, Yiding.

[5]Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre-harvest sprouting and photo-oxidation in rice. Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Sun, Changhui,Liu, Min,Wang, Yiqin,Cheng, Zhukuan,Chu, Chengcai,Fang, Jun,Chai, Chenglin,Li, Chunlai,Tang, Jiuyou,Huang, Zejun,Guo, Xiaoli,Qian, Qian,Sun, Lei,Zhang, Yan,Lu, Qingtao,Lu, Congming,Han, Bin,Chen, Fan.

[6]Isolation and identification of a wheat gene encoding a zinc finger protein (TaZnFP) responsive to abiotic stresses. Min, Dong-Hong,Zhao, Yue,Huo, Dong-Ying,Li, Lian-Cheng,Chen, Ming,Xu, Zhao-Shi,Ma, You-Zhi.

[7]Isolation and functional characterization of HvDREB1-a gene encoding a dehydration-responsive element binding protein in Hordeum vulgare. Xu, Zhao-Shi,Ni, Zhi-Yong,Li, Zhi-Yong,Li, Lian-Cheng,Chen, Ming,Gao, Dong-Yao,Yu, Xiu-Dao,Liu, Pei,Ma, You-Zhi.

[8]Expression of SbSNAC1, a NAC transcription factor from sorghum, confers drought tolerance to transgenic Arabidopsis. Lu, Min,Zhang, Deng-Feng,Shi, Yun-Su,Song, Yan-Chun,Wang, Tian-Yu,Li, Yu,Lu, Min.

[9]Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis. Zhang, Yang,Chen, Chen,Jin, Xiao-Fen,Xiong, Ai-Sheng,Peng, Ri-He,Yao, Quan-Hong,Zhang, Yang,Chen, Chen,Hong, Yi-Huan,Chen, Jian-Min.

[10]Characterization of the ZmCK1 Gene Encoding a Calcium-Dependent Protein Kinase Responsive to Multiple Abiotic Stresses in Maize. Wang, Chang-Tao,Shao, Jun-Ming.

[11]Physiological Investigation and Transcriptome Analysis of Polyethylene Glycol (PEG)-Induced Dehydration Stress in Cassava. Fu, Lili,Ding, Zehong,Han, Bingying,Hu, Wei,Li, Yajun,Zhang, Jiaming. 2016

[12]Characterization of the calcineurin B-Like (CBL) gene family in maize and functional analysis of ZmCBL9 under abscisic acid and abiotic stress treatments. Zhang, Fan,Li, Li,Jiao, Zhenzhen,Chen, Yangsong,Liu, Hui,Chen, Xunji,Fu, Junjie,Wang, Guoying,Zheng, Jun,Zhang, Fan,Li, Li,Jiao, Zhenzhen,Chen, Yangsong,Liu, Hui,Chen, Xunji,Fu, Junjie,Wang, Guoying,Zheng, Jun,Zhang, Fan,Chen, Xunji.

[13]A novel DREB transcription factor from Halimodendron halodendron leads to enhance drought and salt tolerance in Arabidopsis. Ma, J. -T.,Wang, Z. -L.,Ma, J. -T.,Yin, C. -C.,Guo, Q. -Q.,Zhou, M. -L.,Wu, Y. -M.,Guo, Q. -Q..

[14]Functional Characterization of TaSnRK2.8 Promoter in Response to Abiotic Stresses by Deletion Analysis in Transgenic Arabidopsis. Zhang, Hongying,Zhang, Hongying,Jing, Ruilian,Mao, Xinguo. 2017

[15]The Expression Profiling of the Lipoxygenase (LOX) Family Genes During Fruit Development, Abiotic Stress and Hormonal Treatments in Cucumber (Cucumis sativus L.). Yang, Xue-Yong,Jiang, Wei-Jie,Yu, Hong-Jun. 2012

[16]Enhanced expression of a thaumatin-like gene, involved in Pseudoperonospora cubensis and abiotic stresses, induced by DNA introgression from a wild relative, Cueumis hystrix. Wan, Hongjian,Chen, Jinfeng,Wan, Hongjian. 2013

[17]Genome-wide characterization and expression profiling of the NAC genes under abiotic stresses in Cucumis sativus. Zhang, Xiao Meng,Yu, Hong Jun,Sun, Chao,Deng, Jie,Zhang, Xue,Liu, Peng,Li, Yun Yun,Li, Qiang,Jiang, Wei Jie,Sun, Chao,Jiang, Wei Jie.

[18]The abiotic stress-responsive NAC transcription factor SlNAC11 is involved in drought and salt response in tomato (Solanum lycopersicum L.). Lingling Wang,Chen, Guoping,Zongli Hu,Mingku Zhu,Zhiguo Zhu,Jingtao Hu,Ghulam Qanmber,Guoping Chen.

[19]Comparative expression analysis of five WRKY genes from Tibetan hulless barley under various abiotic stresses between drought-resistant and sensitive genotype. Li, Huie,Lan, Xiaozhong,Li, Huie,Zhou, Qi,Guo, Qiqiang,Guo, Qiqiang,Wei, Na.

[20]Genome-Wide Study of YABBY Genes in Upland Cotton and Their Expression Patterns under Different Stresses. Zhaoen Yang,Qian Gong,Li, Fuguang,Lingling Wang,Yuying Jin,Jianping Xi,Zhi Li,Wenqiang Qin,Zuoren Yang,Lili Lu,Quanjia Chen,Fuguang Li. 2018

作者其他论文 更多>>

微信小程序