A highly robust and optimized sequence-based approach for genetic polymorphism discovery and genotyping in large plant populations

文献类型： 外文期刊

第一作者： Jiang, Ning

作者： Jiang, Ning;Zhang, Fengjun;Wu, Jinhua;Hu, Xiaohua;Fang, Ou;Luo, Zewei;Chen, Yue;Leach, Lindsey J.;Luo, Zewei;Wang, Di;Zhang, Fengjun

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期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

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收录情况： SCI

摘要： Key message This optimized approach provides both a computational tool and a library construction protocol, which can maximize the number of genomic sequence reads that uniformly cover a plant genome and minimize the number of sequence reads representing chloroplast DNA and rRNA genes. One can implement the developed computational tool to feasibly design their own RAD-seq experiment to achieve expected coverage of sequence variant markers for large plant populations using information of the genome sequence and ideally, though not necessarily, information of the sequence polymorphism distribution in the genome.

分类号： R394

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