Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii

文献类型： 外文期刊

第一作者： Chen, Jia

作者： Chen, Jia;Li, Zhong-Yuan;Liu, Wen-Ge;Zhu, Xing-Quan;Chen, Jia;Petersen, Eskild;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;Toxoplasmosis;TgCDPK1;pVAX-IL-7-IL-15;DNA vaccine;Protective immunity

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Toxoplasma gondii is an obligatory intracellular parasite, which can infect all warm-blooded animals including humans. Cytokines, including IL-15 and IL-7, play a critical role in the regulation of the homeostasis of naive and memory T cells. Co-administration the DNA vaccine with cytokines may improve its efficacy. IL-7 and IL-15 from splenic tissues of Kunming mice were cloned, and eukaryotic plasmid pVAX-IL-7-IL-15 was constructed. Kunming mice were administrated with DNA vaccine expressing T gondii calcium-dependent protein kinase 1 (TgCDPK1), pVAX-CDPK1, in the presence or absence of IL-7 and IL-15 plasmids (pVAX-IL-7-IL-15), immune responses were analyzed including lymphoproliferative assay, cytokine and serum antibody measurements, flow cytometric surface markers on lymphocytes, and thus protective immunity against acute and chronic T. gondii infection was estimated. Mice injected with pVAX-CDPK1 supplemented with pVAX-IL-7-IL-15 showed higher Toxoplasma-specific IgG2a titers, Th1 responses associated with the production of IFN-gamma, IL-2 as well as cell-mediated cytotoxic activity where stronger frequencies of IFN-gamma secreting CD8+ and CD4+ T cells (CD8+/CD4+ IFN-gamma+ T cells) compared to controls. Co-administration of pVAX-IL-7-IL-15 and pVAX-CDPK1 significantly (P < 0.05) increased survival time (18.07 +/- 5.43 days) compared with pVAX-CDPK1 (14.13 +/- 3.85 days) or pVAX-IL-7-IL-15 (11.73 +/- 1.83 days) alone, and pVAX-IL-7-IL-15 + pVAX-CDPK1 significantly reduced the number of brain cysts (73.5%) in contrast to pVAX-CDPK1 (46.0%) or pVAX-IL-7-IL-15 alone (45.0%). Our results indicate that supplementation of DNA vaccine with IL-7 and IL-15 would facilitate specific humoral and cellular immune responses elicited by DNA vaccine against acute and chronic T. gondii infection in mice. (C) 2015 Elsevier Inc. All rights reserved.

分类号： Q958.9

  • 相关文献

[1]Protective efficacy of Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) adjuvated with recombinant IL-15 and IL-21 against experimental toxoplasmosis in mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhou, Dong-Hui,Zhu, Xing-Quan,Chen, Jia,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild. 2014

[2]Protective immunity induced by a DNA vaccine expressing eIF4A of Toxoplasma gondii against acute toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Li, Zhong-Yuan,Yuan, Zi-Guo,Zhou, Dong-Hui,Zhang, Nian-Zhang,Zhu, Xing-Quan,Chen, Jia,Li, Zhong-Yuan,Yuan, Zi-Guo,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[3]Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice. Li, Zhong-Yuan,Chen, Jia,Zhou, Dong-Hui,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild.

[4]DNA vaccination with genes encoding Toxoplasma gondii antigens ROP5 and GRA15 induces protective immunity against toxoplasmosis in Kunming mice. Chen, Jia,Li, Zhong-Yuan,Huang, Si-Yang,Zhou, Dong-Hui,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan,Petersen, Eskild,Zhu, Xing-Quan. 2015

[5]DNA immunization with eukaryotic initiation factor-2 alpha of Toxoplasma gondii induces protective immunity against acute and chronic toxoplasmosis in mice. Chen, Jia,Huang, Si-Yang,Zhou, Dong-Hui,Li, Zhong-Yuan,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Zhu, Xing-Quan. 2013

[6]Protective immunity against Toxoplasma gondii induced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3. Zhang, Nian-Zhang,Huang, Si-Yang,Zhou, Dong-Hui,Chen, Jia,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan,Tian, Wei-Peng,Lu, Jing,Zhu, Xing-Quan. 2013

[7]Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2). Chen, Kai,Wang, Jin-Lei,Huang, Si-Yang,Yang, Wen-Bin,Zhu, Wei-Ning,Zhu, Xing-Quan,Huang, Si-Yang,Zhu, Xing-Quan,Yang, Wen-Bin,Zhu, Wei-Ning. 2017

[8]Evaluation of protective immunity induced by DNA vaccination with genes encoding Toxoplasma gondii GRA17 and GRA23 against acute toxoplasmosis in mice. Zhu, Wei-Ning,Wang, Jin-Lei,Chen, Kai,Yue, Dong-Mei,Zhang, Xiao-Xuan,Huang, Si-Yang,Zhu, Xing-Quan,Zhu, Wei-Ning. 2017

[9]Evaluation of immuno-efficacy of a novel DNA vaccine encoding Toxoplasma gondii rhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model. Xu, Ying,Zhang, Nian-Zhang,Tan, Qi-Dong,Chen, Jia,Lu, Jing,Zhu, Xing-Quan,Xu, Ying,Tan, Qi-Dong,Xu, Qian-Ming,Zhu, Xing-Quan. 2014

[10]Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice. Yang, Wen-Bin,Zhao, Guang-Hui,Yang, Wen-Bin,Zhou, Dong-Hui,Zou, Yang,Chen, Kai,Wang, Jin-Lei,Zhu, Xing-Quan,Liu, Qing,Zhu, Xing-Quan. 2017

[11]Evaluation of protective effect of pVAX-TgMIC13 plasmid against acute and chronic Toxoplasma gondii infection in a murine model. Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Zhou, Yang,Zhu, Xing-Quan,Yuan, Zi-Guo,Ren, Di,Zhang, Xiu-Xiang,Li, Xiu-Zhen,Petersen, Eskild,Petersen, Eskild,Yang, Gui-Lian,Zhu, Xing-Quan. 2013

[12]A long-lasting protective immunity against chronic toxoplasmosis in mice induced by recombinant rhoptry proteins encapsulated in poly (lactide-co-glycolide) microparticles. Xu, Ying,Zhang, Nian-Zhang,Wang, Meng,Dong, Hu,Feng, Sheng-Yong,Guo, Hui-Chen,Zhu, Xing-Quan,Xu, Ying,Zhu, Xing-Quan.

[13]Protective efficacy of two novel DNA vaccines expressing Toxoplasma gondii rhomboid 4 and rhomboid 5 proteins against acute and chronic toxoplasmosis in mice. Zhang, Nian-Zhang,Xu, Ying,Wang, Meng,Chen, Jia,Huang, Si-Yang,Zhu, Xing-Quan,Xu, Ying,Petersen, Eskild,Huang, Si-Yang,Zhu, Xing-Quan. 2015

[14]DNA vaccination with a gene encoding VP60 elicited protective immunity against rabbit hemorrhagic disease virus. Yuan, Dongwei,Qu, Liandong,Liu, Jiasen,Guo, Dongchun,Jiang, Qian,Lin, Huan,Si, Changde.

[15]Toxoplasma gondii: Protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis. Chen, Jia,Zhou, Dong-Hui,Li, Zhong-Yuan,Huang, Si-Yang,Song, Hui-Qun,Zhu, Xing-Quan,Petersen, Eskild,Petersen, Eskild,Li, Zhong-Yuan,Zhu, Xing-Quan. 2014

[16]Sequence variation in the Toxoplasma gondii ROP20 gene among strains from different hosts and geographical locations. Ning, H. R.,Zhu, X. Q.,Ning, H. R.,Wang, J. L.,Qin, S. Y.,Huang, S. Y.,Lou, Z. L.,Hu, L. Y.,Zhu, X. Q.,Qin, S. Y.,Lou, Z. L.,Huang, S. Y.,Zhu, X. Q.. 2015

[17]Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Xu, Min-Jun,Zhou, Dong-Hui,Huang, Si-Yang,Fan, Yi-Fan,Zhu, Xing-Quan,Nisbet, Alasdair J.,Zhu, Xing-Quan,Fan, Yi-Fan. 2013

[18]Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations. Ren, Di,Zhou, Yang,Lin, Ze-Ping,Lin, Rui-Qing,Wu, Song-Ming,Lin, Shao-Qi,Yuan, Zi-Guo,Ren, Di,Zhou, Dong-Hui,Xu, Min-Jun,Zhou, Yang,Wu, Song-Ming,Yuan, Zi-Guo,Yang, Jian-Fa,Zou, Feng-Cai. 2012

[19]Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP. Jiang, Hai-Hai,Huang, Si-Yang,Zhou, Dong-Hui,Zhang, Xiao-Xuan,Su, Chunlei,Zhu, Xing-Quan,Deng, Shun-Zhou,Su, Chunlei,Zhang, Xiao-Xuan. 2013

[20]Sequence Variation in Rhoptry Neck Protein 10 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. Zhao, Yu,Sun, Xiaolin,Zhao, Yu,Zhou, Donghui,Chen, Jia. 2017

作者其他论文 更多>>

微信小程序