Alanine-Scanning Mutational Analysis of Durancin GL Reveals Residues Important for Its Antimicrobial Activity

文献类型： 外文期刊

第一作者： Ju, Xingrong

作者： Ju, Xingrong;Chen, Xinquan;Du, Lihui;Wu, Xueyou;Yuan, Jian;Liu, Fang

作者机构：

关键词： durancin GL;alanine-scanning mutation;antilisteria;Enterococcus

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Durancin GL is a novel class IIa bacteriocin with 43 residues produced by Enterococcus durans 41D. This bacteriocin demonstrates narrow inhibition spectrum and potent antimicrobial activity against several Listeria monocytogenes strains, including nisin-resistant L. monocytogenes NR30. A systematic alanine-scanning mutational analysis with site-directed mutagenesis was performed to analyze durancin GL residues important for antimicrobial activity and specificity. Results showed that three mutations lost their antimicrobial activity, ten mutations demonstrated a decreased effect on the activity, and seven mutations exhibited relatively high activity. With regard to inhibitory spectrum, four mutants demonstrated a narrower antimicrobial spectrum than wild-type durancin GL. Another four mutants displayed a broader target cell spectrum and increased potency relative to wild-type durancin GL. These findings broaden our understanding of durancin GL residues important for its antimicrobial activity and contribute to future rational design of variants with increased potency.

分类号： R15`S

  • 相关文献
作者其他论文 更多>>
  • Health assessment based on exposure to microplastics in tropical agricultural soil

    作者：Lin, Bigui;Wang, Luya;Liu, Beibei;Liu, Fang;Wu, Lin;Wei, Chaoxian;Lin, Bigui;Wang, Luya;Liu, Beibei;Wu, Lin;Wei, Chaoxian;Lin, Bigui;Wang, Luya;Liu, Beibei;Wu, Lin;Wei, Chaoxian;Chen, Qiyu;Liu, Zhilei;Chen, Xichao;Zhang, Zongyao;Wen, Shaobai;Liu, Fang

    关键词：Microplastics; Agricultural soil; Size distribution analysis; Dimensionality of MPs; Health exposure assessment

  • Widespread incomplete lineage sorting and introgression shaped adaptive radiation in the Gossypium genus

    作者：Xu, Yanchao;Zhou, Zhongli;Cai, Xiaoyan;Umer, Muhammad Jawad;Hou, Yuqing;Wang, Yuhong;Wang, Kunbo;Yu, Shuxun;Liu, Fang;Xu, Yanchao;Wei, Yangyang;Liu, Yuling;Peng, Renhai;Xu, Yanchao;Cai, Xiaoyan;Boden, Scott A.;Safdar, Luqman B.;Jin, Dingsha;Wall, Sarah Brooke;Zhang, Baohong;Liu, Fang

    关键词：cotton speciation; Gossypium genus; incomplete lineage sorting; ILS; phylogenetic analysis; gene tree resolution

  • MCSS: microbial community simulator based on structure

    作者：Hui, Xingqi;Liu, Fang;Hui, Xingqi;Yang, Jinbao;Pan, Weihua;Yang, Jinbao;Sun, Jinhuan;Liu, Fang

    关键词：metagenome; microbiome communities; long reads; simulator; assembly

  • Linkage and association mapping in multi-parental populations reveal the genetic basis of carotenoid variation in maize kernels

    作者：Yin, Pengfei;Fu, Xiuyi;Feng, Haiying;Yang, Yanyan;Xu, Jing;Zhang, Xuan;Wang, Min;Ji, Shenghui;Zhao, Binghao;Fang, Hui;Du, Xiaoxia;Li, Yaru;Hu, Shuting;Li, Kun;Xu, Shutu;Li, Zhigang;Li, Jiansheng;Yang, Xiaohong;Yin, Pengfei;Fu, Xiuyi;Feng, Haiying;Yang, Yanyan;Xu, Jing;Zhang, Xuan;Wang, Min;Ji, Shenghui;Zhao, Binghao;Fang, Hui;Du, Xiaoxia;Li, Yaru;Hu, Shuting;Li, Kun;Xu, Shutu;Li, Zhigang;Li, Jiansheng;Yang, Xiaohong;Fu, Xiuyi;Wang, Yuandong;Liu, Fang;Li, Jiansheng;Yang, Xiaohong;Xiao, Yingni;Yang, Xiaohong

    关键词：Carotenoids; Linkage analysis; Association mapping; Plastid terminal oxidase

  • Genetic Analysis of Cotton Fiber Traits in Gossypium Hybrid Lines

    作者：Wang, Heng;Cai, Xiaoyan;Umer, Muhammad Jawad;Xu, Yanchao;Hou, Yuqing;Zheng, Jie;Liu, Fang;Wang, Kunbo;Zhou, Zhongli;Chen, Mengshan;Ma, Shuping;Yu, Jingzhong;Cai, Xiaoyan;Zheng, Jie;Liu, Fang;Liu, Fang;Cai, Xiaoyan;Hou, Yuqing;Zheng, Jie;Liu, Fang

    关键词：

  • Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution

    作者：Li, Pengtao;Liu, Qiankun;Wei, Yangyang;Liu, Yuling;Lu, Quanwei;Hu, Nan;Wang, Tao;Cheng, Shuang;Li, Zhaoguo;Zhao, Zilin;Li, Yanfang;Peng, Renhai;Li, Pengtao;Liu, Qiankun;Xing, Chaozhu;Han, Jiangping;Cai, Xiaoyan;Zhou, Zhongli;Wang, Kunbo;Liu, Fang;Li, Pengtao;Xing, Chaozhu;Liu, Fang;Peng, Renhai;Liu, Qiankun;Cheng, Shuang;Li, Zhaoguo;Peng, Renhai;Xu, Zhongping;Zhu, Xiangqian;Jin, Shuangxia;Ding, Fang;Zhang, Baohong

    关键词：cotton; salt stress; scRNA-seq; root cell types; virus-induced gene silencing

  • Assessment of benthic ecological status and heavy metal contamination in an estuarine intertidal mudflat in the Northern Bohai Sea

    作者：Li, Ang;Li, Jiaqi;Liu, Lulei;Xue, Suyan;Zhang, Meng;Tang, Yuze;Mao, Yuze;Li, Ang;Li, Jiaqi;Zhu, Ling;Liu, Lulei;Xue, Suyan;Zhang, Meng;Tang, Yuze;Mao, Yuze;Liu, Fang

    关键词：Geligang; Macrobenthos; Benthic ecological quality; M-AMBI; Heavy metals

微信小程序