Stereoselective Determination of Tebuconazole in Water and Zebrafish by Supercritical Fluid Chromatography Tandem Mass Spectrometry

文献类型: 外文期刊

第一作者: Liu, Na

作者: Liu, Na;Dong, Fengshou;Xu, Jun;Liu, Xingang;Chen, Zenglong;Tao, Yan;Pan, Xinglu;Chen, XiXi;Zheng, Yongquan

作者机构:

关键词: tebuconazole;chiral separation;supercritical fluid chromatography-tandem mass spectrometry;zebrafish

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A simple and sensitive method for the enantioselective determination of tebuconazole enantiomers in water and zebrafish has been established using supercritical fluid Chromatography (SFC)-MS/MS. The effects of the chiral stationary phases, mobile phase, auto back pressure regulator (ABPR) pressure, column temperature, flow rate of the mobile phase, and compensation pump solvent were evaluated. Finally, the optimal SFC-MS/MS working conditions were determined to include a CO2/MeOH mobile phase (87:13, v/v), 2.0 mL/min flow rate, 2200 psi ABPR, and 30 degrees C column temperature using a Chiralpak IA-3 chiral column under electrospray ionization positive mode. The modified QuEChERS method was applied to water and zebrafish samples. The mean recoveries for the tebuconazole enantiomers were 79.8-108.4% with RSDs <= 7.0% in both matrices. The LOQs ranged from 0.24 to 1.20 mu g/kg. The developed analytical method was further validated by application to the analysis of authentic samples.

分类号: R15`S

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