Separation and quantification of milk casein from different buffalo breeds

文献类型： 外文期刊

第一作者： Li, Shanshan

作者： Li, Shanshan;Liu, Jianxin;Ren, Daxi;Li, Ling;Zeng, Qingkun;Li, Ling

作者机构：

关键词： Swamp buffalo;river buffalo;crossbreed;milk protein;comparative proteomics

期刊名称：JOURNAL OF DAIRY RESEARCH （ 影响因子：1.904； 五年影响因子：2.019 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Understanding the milk protein expression profile in different buffalo breeds plays an important role in improving hybrid selection and determining the effects on milk protein synthesis. The aim of this research is to compare the differences in milk protein content, composition and distribution between River buffalo and their crossbreeds for hybrid screening. Four groups of milk samples that included Nili-Ravi (N), Murrah (M), a Nili-Ravi-Murrah crossbreed (M-N), and a crossbreed of river buffalo with local swamp buffalo (C) were collected. The protein composition of the buffalo milk was determined by RP-HPLC. A gel-based proteomic approach consisting of two-dimensional gel electrophoresis coupled with mass spectrometry was utilised for the detailed protein characterisation of milk from different breeds. The results of this analysis showed that the river/swamp buffalo crossbreed (C) displayed the highest content of total protein (446%) and -casein (1114%) but the lowest content of -lactalbumin (679%). By selecting 23 different protein spots among the four types of milk that contained the most spots corresponding to -casein, -casein and (s1)-casein, correlations between the crossbreeds, protein polymorphism and phosphorylation could be made. The results of this study indicate that crossbreeding a swamp buffalo with a river buffalo has a notable effect on the protein content and composition that may be exploited for producing high-quality raw milk in food technology applications and dairy food production.

分类号： TS252

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