Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells
文献类型: 外文期刊
第一作者: Liu, Qingtao
作者: Liu, Qingtao;Huang, Xinmei;Zhao, Dongmin;Han, Kaikai;Liu, Yuzhuo;Yang, Jing;Bi, Keran;Li, Yin
作者机构:
关键词: Tembusu virus;Heat shock protein A9;Receptor
期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )
ISSN:
年卷期:
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收录情况: SCI
摘要: This study attempts to identify receptor elements for Tembusu virus (TMUV) on DF-1 cells. Using coimmunoprecipitation and virus overlay protein binding assays, we identified a TMUV-binding protein of approximately 70-kDa on DF-1 cell membranes. Mass spectroscopy identified the protein to be heat shock protein (HSP) A9, which was reconfirmed by an anti-HSPA9 antibody. Indirect immunofluorescence demonstrated a significant degree of colocalization between HSPA9 and TMUV on cell surface. Additionally, an antibody against HSPA9 could inhibit TMUV infection in DF-1 cells in a dose-dependent manner. These results might suggest that HSPA9 is a putative receptor for TMUV. (C) 2016 Elsevier B.V. All rights reserved.
分类号: R37
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