Heterologous production of an acidic thermostable lipase with broad-range pH activity from thermophilic fungus Neosartorya fischeri P1
文献类型: 外文期刊
第一作者: Sun, Qiaoqiao
作者: Sun, Qiaoqiao;Wang, Hui;Luo, Huiying;Shi, Pengjun;Bai, Yingguo;Yao, Bin;Huang, Huoqing;Sun, Qiaoqiao;Zhang, Huitu;Lu, Fuping
作者机构:
关键词: Lipase;Broad pH adaptability;Thermophilic fungus;Pichia pastoris;Neosartorya fischeri
期刊名称:JOURNAL OF BIOSCIENCE AND BIOENGINEERING ( 影响因子:2.894; 五年影响因子:2.746 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Thermophilic Neosartorya fischeri P1 is an excellent lipase producer and harbors seven lipase genes. All genes were found to be functional after heterologous expression in Escherichia coli. One of them, LIP09, showed high-level expression in Pichia pastoris with the yield of 2.0 g/L in a 3.7-L fermentor. Deduced amino acid sequence of LIP09 consists of a putative signal peptide (residues 1-19) and a mature polypeptide (residues 20-562). Compared with other fungal counterparts, purified recombinant LIP09 has some superior properties. It exhibited maximum activity at 60 degrees C and pH 5.0, had broad pH adaptability (>60% activity at pH 3.5-8.0) and stability (retaining >90% activity after incubation at pH 3.0-7.0 for 1 hat 40 degrees C), and was highly thermostable (retaining >96% activity after incubation at 50 degrees C for 30 min). The r-LIP09 had a preference for the medium-chain length p-nitrophenyl esters (C12) rather than short and long-chain length substrates. The high-level expression and excellent properties make LIP09 a potential enzyme candidate in food and feed industries. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.
分类号: Q5`Q81`TS26
- 相关文献
作者其他论文 更多>>
-
Controllable self-cleaning FET self-assembled RNA-cleaving DNAzyme based DNA nanotree for culture-free Staphylococcus aureus detection
作者:Wang, Hui;Chen, Ruipeng;He, Yue;Zhu, Xiaoyan;Yu, Zhixue;Pan, Dongxia;Yang, Liang;Tang, Xiangfang;Xiong, Benhai;Feng, Zemeng;He, Yue
关键词:Self-cleaning field effect transistor; DNA origami; Electrochemical biosensor; Carbon nanotube; Superhydrophobic-oleophobic coating
-
Humoral and cellular immune responses following Omicron BA.2.2 breakthrough infection and Omicron BA.5 reinfection
作者:Zhao, Xin-Jing;Fang, Li-Qun;Zhao, Xin-Jing;Li, De-Yu;Zhang, Sheng;Gu, Hong-Jing;Chen, Jin-Jin;Xu, Qiang;Lv, Chen-Long;Jiang, Bao-Gui;Wang, Hui;Wang, Guo-Lin;Fang, Li-Qun;Ji, Bin;Peng, Hong-Hong;Shi, Chao;Shen, Yuan;Shang, Chao;Zhang, Cui-Ling;Li, Xiao;Qian, Cheng
关键词:
-
Unraveling the regulatory network of miRNA expression in Potato Y virus-infected of Nicotiana benthamiana using integrated small RNA and transcriptome sequencing
作者:Song, Hongping;Zhang, Songbai;Gao, Xinwen;Song, Liyun;Jiao, Yubing;Shen, Lili;Yang, Jinguang;Li, Ying;Li, Changquan;Shang, Jun;Wang, Hui
关键词:high-throughput sequencing; miRNA; Potato virus Y; miRNA-mRNA network; viral infection
-
High-Throughput Screening Techniques for the Selection of Thermostable Enzymes
作者:Li, Lanxue;Bai, Yingguo;Yao, Bin;Luo, Huiying;Tu, Tao;Liu, Xiaoqing
关键词:high-throughput screening; virtual screening; protein engineering; enzyme thermostability
-
Recombinant production of SAG1 fused with xylanase in Pichia pastoris induced higher protective immunity against Eimeria tenella infection in chicken
作者:Liu, Chen;Wei, HanBing;Wang, Yuan;Su, Xiaoyun;Tu, Tao;Luo, Huiying;Yao, Bin;Huang, Huoqing;Zhang, Honglian;Liang, Ruiying;Ding, Jiabo;Tang, Xinming
关键词:
-
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
作者:He, Jinjian;Li, Yuying;Sun, Xihang;Zuo, Dinghui;Wang, Mansheng;Zheng, Xia;Shi, Pengjun;Zuo, Dinghui;Yu, Pinglian
关键词:beta-glucosidase; Acidilobus sp.; thermophilic; thermostability; ethanol tolerance; isoflavone glycosides
-
Porphyrin-based covalent organic framework as oxidase mimic for highly sensitive colorimetric detection of pesticides
作者:Liu, Qingju;Zhu, Junyi;Wang, Hui;Luan, Yunxia;Zhang, Zhikun
关键词:Porphyrin-based covalent organic framework; Oxidase mimics; Colorimetric detection; Pesticides