Mapping and expression profiling reveal an inserted fragment from purple mustard involved anthocyanin accumulation in Chinese cabbage

文献类型： 外文期刊

第一作者： Zhang, Shujiang

作者： Zhang, Shujiang;Li, Peirong;Qian, Wei;Zhang, Shifan;Li, Fei;Zhang, Hui;Wang, Xiaowu;Sun, Rifei

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关键词： Mapping;Expression analysis;HPLCMS;Purple mustard fragment;Chinese cabbage

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

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收录情况： SCI

摘要： Anthocyanins are the major pigments responsible for purple coloration in flowers, fruits and leaves, and the genes involved in their biosynthetic pathway have been identified in many plants. A purple-leaf Chinese cabbage (Brassica rapa L. ssp. pekinensis) was bred by interspecies crossing between Chinese cabbage and purple-leaf mustard [Brassica juncea (L.) Coss. var. foliosa L. H. Bailey]. In this study, high-performance liquid chromatographic analysis indicated purple coloration in Chinese cabbage is due to the accumulation of the same kind of cyaninin as in purple mustard. To elucidate the genetic factors controlling anthocyanin accumulation in this purple-leaf Chinese cabbage, we mapped the anthocyanin gene from the mustard (Anm) locus in an F-2 population and performed expression profiling of anthocyanin-related genes. A genetic analysis revealed that the purple-leaf phenotype is a qualitative trait and that its inheritance is unstable in purple-leaf Chinese cabbage. Mapping insertion/deletion markers from 288 individuals of the F-2 population located the Anm locus within a 2.5-cM interval on B. rapa chromosome A02. The sequencing and alignment of the amplified fragments demonstrated that purple Chinese cabbage contains fragments of purple mustard on chromosome A02. We evaluated the expression profiles of 12 anthocyanin-related genes on A02 by reverse-transcription and quantitative real-time PCR methods, which revealed that the expression levels of five genes were higher in purple Chinese cabbage than in the non-purple variety. These results offer insights into the molecular mechanism of anthocyanin biosynthesis and improve the knowledge on molecular breeding of purple-type Chinese cabbage.

分类号： S3

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