Cloning and characterization of Tabas1-B1 gene associated with flag leaf chlorophyll content and thousand-grain weight and development of a gene-specific marker in wheat

文献类型: 外文期刊

第一作者: Zhu, Xiao-Feng

作者: Zhu, Xiao-Feng;Zhang, Hai-Ping;Hu, Ming-Jian;Wu, Zeng-Yun;Jiang, Hao;Cao, Jia-Jia;Xia, Xian-Chun;Ma, Chuan-Xi;Chang, Cheng;Xia, Xian-Chun

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关键词: Functional marker;Chlorophyll content;Linkage and association analysis;Tabas1-B1 clone;Triticum aestivum L.

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

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收录情况: SCI

摘要: The chloroplast photosystem of flag leaves contributes the largest proportion of photosynthates to grain in crops and consequently affects grain weight. The plant 2-Cys peroxiredoxin BAS1 is involved in chlorophyll protection against chloroplast damage. In the present study, we cloned a Tabas1 gene in common wheat (Triticum aestivum L.), comprising seven exons and six introns with a complete sequence of 2847 bp and an open reading frame of 789 bp. The gene was located on chromosome 2B, and designated Tabas1-B1. A codominant gene-specific marker TaS1 was developed based on a 1-bp InDel (-/A) in the second intron of Tabas1-B1. Two alleles, Tabas1-B1a and Tabas1-B1b, at the Tabas1-B1 locus were identified by TaS1. Linkage and quantitative trait locus (QTL) mapping indicated that Tabas1-B1 was linked to Xcfa2278 (5.23 cM) and Xbarc167 (10.38 cM) on chromosome 2BL. A stable QTL co-segregating with Tabas1-B1 explained 9.0-19.2 % of phenotypic variations for chlorophyll content (ChlC) and 9.5-15.5 % for thousand-grain weight (TGW), respectively, across three environments. Association analysis further indicated a significant and positive effect of Tabas1-B1a on the ChlC of flag leaf post-anthesis and TGW in two populations across four environments. Geographic distribution analysis suggested a slightly higher frequency of Tabas1-B1a than Tabas1-B1b in the main wheat-growing regions of China. Selection of Tabas1-B1a may increase grain weight in wheat breeding.

分类号: Q94

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