Proteomics Changes in Filling Seeds of Vegetable Soybean

文献类型: 外文期刊

第一作者: Zhang, Yumei

作者: Zhang, Yumei;Hu, Runfang;Li, Huawei;Zhu, Haisheng;Lin, Guoqiang;Zhao, Jinming;Guo, Na;Xing, Han

作者机构:

关键词: proteomics;2-DE;LC-ESI-MS/MS;seed filling;Glycine max

期刊名称:HORTSCIENCE ( 影响因子:1.455; 五年影响因子:1.617 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Vegetable soybean is an important economic and nutritious crop. In this study, 48 differentially expressed proteins were identified from filling seeds of soybean (Glycine max) cv. Mindou 6 by using two-dimensional electrophoresis (2-DE) combined with liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Among them, 25% were related to protein destination and storage, 42% to energy and metabolism, 15% to disease/defense, 6% to transporters, 4% to secondary metabolism, 4% to transcription, 2% to protein synthesis, and 2% to cell growth/division. Along with the maturity of seeds, the number of unchanged abundance proteins decreased, while that of both upregulated and downregulated proteins increased. Both downregulated expression of caffeic acid O-methyltransferase (COMT) and upregulated expression of sucrose-binding protein (SBP) 2 precursor may contribute to increase in digestibility, nutritional value, and eating quality of vegetative seeds at suitable picking period. The pattern of unchanged proteins during the whole seed-filling stage may be also beneficial to the quality of vegetable soybean.

分类号: S6

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