A simple and efficient in planta transformation method for pommelo (Citrus maxima) using Agrobacterium tumefaciens
文献类型: 外文期刊
第一作者: Zhang, Yong-yan
作者: Zhang, Yong-yan;Zhang, Dong-min;Chang, Xiao-jun;Cheng, Chun-zhen;Zhong, Yun;Hu, Min-lun
作者机构:
关键词: Agrobacterium-mediated transformation;Citrus maxima;Dark incubation;in planta transformation
期刊名称:SCIENTIA HORTICULTURAE ( 影响因子:3.463; 五年影响因子:3.672 )
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年卷期:
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收录情况: SCI
摘要: A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integration confirmation and transgenic plant propagation. The highest transformation efficiency (20.41%) was obtained when using hygromycin as selection marker, followed by Basta (1937%) and Kanamycin (3.21%). Trans genic plants obtained using this method could be graft-propagated in 3 months post transformation, which is shorter than the tissue-culture-dependent transformation. The transformation protocol presented here has great potential to be used for citrus genetic breeding and improvement. (C) 2016 Elsevier B.V. All rights reserved.
分类号: S6
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